Wei Bo, Ta Na, Li Jia, Zhang Jian-fang, Chen Bi-liang
Department of Gynecology and Obstetrics, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010 Mar;26(3):235-7, 241.
To observe the expression of nuclear factor-kappaB (NF-kappaB) and glucose transporter-4 (GLUT-4) in uteru of rats with gestational diabetes mellitus (GDM) and to explore the relationship between inhibition of NF-kappaB and insulin resistance.
Thirty Sprague-Dawley rats were randomly divided into 3 groups: normal pregnant control (NC) group, GDM group and PDTC group, 10 rats each group. GDM rat model was established by intraperitoneal (ip) injection of streptozotocin (STZ). PDTC (40 mg/kg) was ip injected in PDTC group.The rats were sacrificed on the 20-day of pregnancy before delivery. The uterus were taken to observe the pathological changes. The expressions of GLUT-4 and NF-kappaB in uteru tissue were detected by immunohistochemical staining and Western blot.
The renal NF-kappaB activity in GDM group was higher significantly than that in NC group (P<0.01); and NF-kappaB activity in PDTC group was lower than that in GDM group (P<0.01). The expression of GLUT-4 in GDM group was significantly lower than that in NC group (P<0.01). However, compared with GDM group, the expression of GLUT-4 in PDTC group was increased (P<0.01).
The inhibition of NF-kappaB activity can increase the expression of GLUT-4 in uterus of GDM rats, indicating that the occurrence of GDM in rats with uteru insulin resistance may be related with the intervention of NF-kappaB activity on the down regulation of the expression of GLUT-4.
观察核因子-κB（NF-κB）和葡萄糖转运蛋白4（GLUT-4）在妊娠期糖尿病（GDM）大鼠子宫中的表达，探讨抑制NF-κB与胰岛素抵抗之间的关系。
将30只Sprague-Dawley大鼠随机分为3组：正常妊娠对照组（NC组）、GDM组和PDTC组，每组10只。通过腹腔注射链脲佐菌素（STZ）建立GDM大鼠模型。PDTC组腹腔注射PDTC（40mg/kg）。在妊娠第20天分娩前处死大鼠。取子宫观察病理变化。采用免疫组织化学染色和Western blot检测子宫组织中GLUT-4和NF-κB的表达。
GDM组肾组织NF-κB活性显著高于NC组（P<0.01）；PDTC组NF-κB活性低于GDM组（P<0.01）。GDM组GLUT-4表达显著低于NC组（P<0.01）。然而，与GDM组相比，PDTC组GLUT-4表达增加（P<0.01）。
抑制NF-κB活性可增加GDM大鼠子宫中GLUT-4的表达，提示子宫胰岛素抵抗的GDM大鼠发病可能与NF-κB活性干预GLUT-4表达下调有关。
