2 型血管紧张素 II 受体在肾上腺中的定位。
Localization of type-2 angiotensin II receptor in adrenal gland.
机构信息
Department of Cell and Systems Physiology, University of Occupational and Environmental Health, Kitakyushu, Japan.
出版信息
J Histochem Cytochem. 2010 Jul;58(7):585-93. doi: 10.1369/jhc.2010.955575. Epub 2010 Mar 15.
The localization of the type-2 angiotensin II receptor (AT2) in the adrenal glands of rats, guinea pigs, bovines, and humans was examined at the mRNA and protein levels. PCR products for AT2 were detected in the adrenal cortices and adrenal medullae of all the mammals examined with an RT-PCR technique. Three different anti-AT2 antibodies (Abs), whose specificity was confirmed in our hands, recognized a 50-kDa protein in the adrenal glands of the four mammals, and this recognition was abolished by the preabsorption of an Ab with an antigen. Immunoblotting and immunohistochemistry revealed that the 50-kDa protein was expressed consistently and variably in the adrenal cortices and medullae of various mammals, respectively. We conclude that the 50-kDa AT2 is consistently expressed in the adrenal cortex in a wide variety of mammals.
在大鼠、豚鼠、牛和人类的肾上腺中，对 2 型血管紧张素 II 受体（AT2）在 mRNA 和蛋白质水平上的定位进行了研究。应用 RT-PCR 技术，在所有检查的哺乳动物的肾上腺皮质和髓质中都检测到 AT2 的 PCR 产物。三种不同的抗 AT2 抗体（Abs）在我们的实验中证实了其特异性，它们在这四种哺乳动物的肾上腺中识别出一种 50kDa 的蛋白质，而这种识别被抗原预吸收的 Ab 所消除。免疫印迹和免疫组织化学显示，50kDa 的蛋白质在各种哺乳动物的肾上腺皮质和髓质中分别一致和不同地表达。我们的结论是，50kDa 的 AT2 在各种哺乳动物的肾上腺皮质中一致表达。
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