Department of Organismic Biology, University of Salzburg, Hellbrunnerstrasse 34, A-5020 Salzburg, Austria.
J Insect Sci. 2006;6:1-7. doi: 10.1673/031.006.4301.
Abstract Patterns of juvenile hormone have been intensively studied in the cockroach Blattella germanica under different physiological situations. However, data have been mainly obtained in vitro, and refer to hormone synthesized by isolated corpora allata, whereas information available on hormone concentration in the hemolymph is restricted to adult females. In order to complement our studies in vitro, we have measured juvenile hormone titer in the hemolymph of B. germanica females in four characteristic physiological situations: penultimate and last instar nymphs, adults during the first vitellogenic cycle, and adults transporting egg cases (ootheca). In general, a significant positive correlation between rates of hormone synthesis and concentration in the hemolymph is observed. The main disparities appear in the penultimate day of the period of ootheca transport, where titer is high whereas synthesis is low, and on day 6 of the first vitellogenic cycle, where synthesis increases whereas titer decreases. At these stages, the observed disparities between synthesis and titer might be explained by differential action of degradation enzymes.
在不同的生理情况下，人们对德国蟑螂 Blattella germanica 中的保幼激素模式进行了深入研究。然而，这些数据主要是在体外获得的，并且指的是由分离的咽下神经节合成的激素，而关于血淋巴中激素浓度的信息仅限于成年雌性。为了补充我们的体外研究，我们测量了 B. germanica 雌性在四个典型生理情况下的血淋巴中保幼激素滴度：倒数第二龄和最后龄若虫、处于第一个卵黄发生周期的成虫以及正在运输卵囊（卵鞘）的成虫。一般来说，我们观察到激素合成率和血淋巴浓度之间存在显著的正相关。主要的差异出现在卵鞘运输的倒数第二天，此时滴度高而合成率低，以及在第一个卵黄发生周期的第 6 天，此时合成率增加而滴度降低。在这些阶段，观察到的合成和滴度之间的差异可能是由于降解酶的不同作用造成的。
