Tobe S S, Ruegg R P, Stay B A, Baker F C, Miller C A, Schooley D A
Experientia. 1985 Aug 15;41(8):1028-34. doi: 10.1007/BF01952127.
Titres of juvenile hormone (JH) have been determined in both hemolymph and whole body extracts of female Diploptera punctata during the first gonotrophic cycle using a method employing gas chromatography/mass spectrometry for qualitative and quantitative analysis. JH III is the sole JH found in both adult and last instar D. punctata. Maximum values of approximately 1500 ng/ml (approximately 6 microM) were observed at the middle of the gonotrophic cycle, when basal oocyte growth rate was greatest. Changes in rates of JH release in vitro by corpora allata paralleled closely the changes in JH titre, suggesting that biosynthesis is a major regulator of titre. JH levels per animal were calculated from observed JH titres, and at certain time points in the gonotrophic cycle JH obtained from analysis of whole bodies were significantly greater than those predicted from hemolymph titres. These results suggest the existence of a nonhemolymph JH pool in D. punctata. Decay in JH titre after allatectomy of 5 day females has also been studied. Following a rapid initial decline, the rate of decay slowed appreciably 4 h post-operation. Thus, use of a first-order rate constant to estimate half-life of JH significantly underestimated the longevity of the hormone. The apparent persistence of JH following allatectomy may be due to the existence of a nonhemolymph JH pool.
采用气相色谱/质谱联用的定性和定量分析方法，测定了雌性澳洲蜚蠊在第一个生殖营养周期中血淋巴和全身提取物中的保幼激素（JH）滴度。JH III是在成年和末龄澳洲蜚蠊中发现的唯一JH。在生殖营养周期中期观察到最大值约为1500 ng/ml（约6 microM），此时基础卵母细胞生长速率最大。咽侧体体外释放JH的速率变化与JH滴度变化密切平行，表明生物合成是滴度的主要调节因素。根据观察到的JH滴度计算每只动物的JH水平，在生殖营养周期的某些时间点，从全身分析获得的JH显著高于从血淋巴滴度预测的值。这些结果表明澳洲蜚蠊中存在非血淋巴JH库。还研究了5日龄雌性蜚蠊切除咽侧体后JH滴度的衰减情况。在最初快速下降之后，术后4小时衰减速率明显减慢。因此，使用一级速率常数来估计JH的半衰期显著低估了该激素的寿命。切除咽侧体后JH的明显持久性可能归因于非血淋巴JH库的存在。
