无血清培养基培养的牙龈成纤维细胞的创伤愈合作用。
Wound healing effects of gingival fibroblasts cultured in animal-free medium.
机构信息
Department of Oral and Maxillofacial Surgery, Division of Cervico-Gnathostmatology, Programs for Applied Biomedicine, Graduate School of Biomedical Sciences, Hiroshima University, Minami-Ku, Hiroshima, Japan.
出版信息
Oral Dis. 2010 Jul;16(5):438-44. doi: 10.1111/j.1601-0825.2010.01654.x. Epub 2010 Mar 9.
OBJECTIVE
The purpose of this study was to develop a graft material made of gingival fibroblasts cultured in animal-free medium (HFDM1).
METHODS
We examined the effects of human serum (HS) on cell growth and wound healing capability, demonstrated by cytokine production, of gingival fibroblasts cultured in HFDM1. Subsequently, the capability of fibroblasts cultured in HFDM1 with 2% HS to promote the healing of skin defects was evaluated using nude mice.
RESULTS
The proliferation of human gingival fibroblasts was increased when HS at a concentration of 0.5-2% was added to HFDM1. Wound healing cytokines, including transforming growth factor-beta, keratinocyte growth factor, hepatocyte growth factor, vascular endothelial growth factor, and IL-6 produced by gingival fibroblasts were increased by adding 2% HS to HFDM1. In addition, gingival fibroblasts cultured in HFDM1 with 2% HS improved wound healing of mouse skin defects as well as those cultured in Dulbecco's modified Eagle's medium with 10% fetal calf serum.
CONCLUSION
Gingival fibroblasts cultured in HFDM1 with 2% HS may be useful as a graft material for reconstruction.
目的
本研究旨在开发一种由无动物源培养基（HFDM1）培养的牙龈成纤维细胞制成的移植物材料（HFDM1）。
方法
我们研究了人血清（HS）对 HFDM1 培养的牙龈成纤维细胞的细胞生长和伤口愈合能力（通过细胞因子产生来证明）的影响。随后，我们使用裸鼠评估了 HFDM1 中添加 2% HS 的成纤维细胞促进皮肤缺损愈合的能力。
结果
当 HFDM1 中添加浓度为 0.5-2%的 HS 时，人牙龈成纤维细胞的增殖增加。向 HFDM1 中添加 2% HS 可增加牙龈成纤维细胞产生的转化生长因子-β、角质形成细胞生长因子、肝细胞生长因子、血管内皮生长因子和白细胞介素-6 等伤口愈合细胞因子。此外，HFDM1 中添加 2% HS 培养的牙龈成纤维细胞可改善小鼠皮肤缺损的愈合，与在添加 10%胎牛血清的杜氏改良 Eagle 培养基中培养的成纤维细胞一样。
结论
HFDM1 中添加 2% HS 的牙龈成纤维细胞可能可用作重建的移植物材料。
Zotero 插件