Biotechnology Institute, Vitoria, Spain.
J Periodontol. 2012 Aug;83(8):1028-37. doi: 10.1902/jop.2011.110505. Epub 2011 Dec 6.
Periodontitis involves inflammation and infection of the ligaments and bones that support the teeth. Gingival fibroblasts are the most abundant cells in periodontal tissue, and they play a role in maintaining the structural integrity of the tissue. Plasma rich in growth factors contain a pool of proteins and growth factors that promote wound healing and tissue regeneration. In the present study, we evaluate the potential of different formulations obtained with this approach to stimulate several biologic processes involved in wound healing, including fibroblast proliferation, migration, adhesion, and the autocrine release of some angiogenic factors and extracellular matrix components. Furthermore, the ability of this technology to prevent and inhibit transforming growth factor β1-induced myodifferentiation was also determined.
Cell proliferation was evaluated through a colorimetric assay, cell migration was performed on culture inserts, and cell adhesion was studied through a fluorescence-based method. Enzyme-linked immunosorbent assay was used to determine some of the biomolecules released by gingival fibroblasts. Smooth muscle actin expression was assessed through immunofluorescence microscopy.
Results showed that plasma rich in growth factors significantly increased gingival fibroblast proliferation, migration, and cell adhesion on type I collagen matrix. In addition, it stimulated the autocrine expression of vascular endothelial growth factor, hepatocyte growth factor, and hyaluronic acid. The myofibroblast phenotype, which is characterized by expressing α-smooth muscle actin, was inhibited and reverted by treating with this technology.
These findings suggest that plasma rich in growth factors is capable of promoting regeneration of gingival connective tissue by stimulating some of the main processes involved in wound regeneration.
牙周炎涉及支持牙齿的韧带和骨骼的炎症和感染。牙龈成纤维细胞是牙周组织中最丰富的细胞，它们在维持组织结构完整性方面发挥作用。富含生长因子的血浆含有促进伤口愈合和组织再生的蛋白质和生长因子池。在本研究中，我们评估了通过这种方法获得的不同配方刺激参与伤口愈合的几种生物学过程的潜力，包括成纤维细胞增殖、迁移、黏附以及某些血管生成因子和细胞外基质成分的自分泌释放。此外，还确定了该技术预防和抑制转化生长因子 β1 诱导的肌分化的能力。
通过比色法评估细胞增殖，在培养插入物上进行细胞迁移，通过基于荧光的方法研究细胞黏附。酶联免疫吸附试验用于测定牙龈成纤维细胞释放的一些生物分子。通过免疫荧光显微镜评估平滑肌肌动蛋白的表达。
结果表明，富含生长因子的血浆显著增加了牙龈成纤维细胞在 I 型胶原基质上的增殖、迁移和细胞黏附。此外，它刺激血管内皮生长因子、肝细胞生长因子和透明质酸的自分泌表达。富含生长因子的血浆处理可抑制和逆转肌成纤维细胞表型，其特征是表达α-平滑肌肌动蛋白。
这些发现表明，富含生长因子的血浆能够通过刺激参与伤口再生的一些主要过程来促进牙龈结缔组织的再生。
