Department of Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, Merelbeke, Belgium.
BMC Genomics. 2010 Mar 16;11:179. doi: 10.1186/1471-2164-11-179.
The ribosomal protein SA (RPSA), previously named 37-kDa laminin receptor precursor/67-kDa laminin receptor (LRP/LR) is a multifunctional protein that plays a role in a number of pathological processes, such as cancer and prion diseases. In all investigated species, RPSA is a member of a multicopy gene family consisting of one full length functional gene and several pseudogenes. Therefore, for studies on RPSA related pathways/pathologies, it is important to characterize the whole family and to address the possible function of the other RPSA family members. The present work aims at deciphering the RPSA family in sheep.
In addition to the full length functional ovine RPSA gene, 11 other members of this multicopy gene family, all processed pseudogenes, were identified. Comparison between the RPSA transcript and these pseudogenes shows a large variety in sequence identities ranging from 99% to 74%. Only one of the 11 pseudogenes, i.e. RPSAP7, shares the same open reading frame (ORF) of 295 amino acids with the RPSA gene, differing in only one amino acid. All members of the RPSA family were annotated by comparative mapping and fluorescence in situ hybridization (FISH) localization. Transcription was investigated in the cerebrum, cerebellum, spleen, muscle, lymph node, duodenum and blood, and transcripts were detected for 6 of the 11 pseudogenes in some of these tissues.
In the present work we have characterized the ovine RPSA family. Our results have revealed the existence of 11 ovine RPSA pseudogenes and provide new data on their structure and sequence. Such information will facilitate molecular studies of the functional RPSA gene taking into account the existence of these pseudogenes in the design of experiments. It remains to be investigated if the transcribed members are functional as regulatory non-coding RNA or as functional proteins.
核糖体蛋白 SA（RPSA），以前称为 37kDa 层粘连蛋白受体前体/67kDa 层粘连蛋白受体（LRP/LR），是一种多功能蛋白，在许多病理过程中发挥作用，如癌症和朊病毒病。在所有研究的物种中，RPSA 是一个由一个全长功能基因和几个假基因组成的多拷贝基因家族的成员。因此，对于研究与 RPSA 相关的途径/病理学，重要的是要描述整个家族，并解决其他 RPSA 家族成员的可能功能。本工作旨在阐明绵羊中的 RPSA 家族。
除了全长功能的绵羊 RPSA 基因外，还鉴定了该多拷贝基因家族的另外 11 个成员，均为加工假基因。RPSA 转录本与这些假基因的比较显示，序列同一性的差异很大，范围从 99%到 74%。在 11 个假基因中，只有一个，即 RPSAP7，与 RPSA 基因具有相同的 295 个氨基酸的开放阅读框（ORF），仅在一个氨基酸上有所不同。RPSA 家族的所有成员均通过比较作图和荧光原位杂交（FISH）定位进行注释。在大脑、小脑、脾脏、肌肉、淋巴结、十二指肠和血液中研究了转录，并且在这些组织中的一些组织中检测到了 6 个假基因的转录物。
在本工作中，我们对绵羊的 RPSA 家族进行了特征描述。我们的结果揭示了 11 个绵羊 RPSA 假基因的存在，并提供了关于它们的结构和序列的新数据。这些信息将有助于在设计实验时考虑到这些假基因的存在，对功能性 RPSA 基因进行分子研究。尚需研究转录成员是否作为功能性非编码 RNA 或功能性蛋白质发挥作用。
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