The University of Queensland, UQ Centre for Clinical Research, Royal Brisbane & Women's Hospital, Brisbane, Australia.
Immun Ageing. 2010 Mar 16;7:4. doi: 10.1186/1742-4933-7-4.
There is reported to be a decline in immune function and an alteration in the frequency of circulating lymphocytes with advancing age. There are also differences in ageing and lifespan between males and females. We performed this study to see if there were differences between males and females in the frequency of the different lymphocyte subsets with age.
Using flow cytometry we have examined different populations of peripheral blood leukocytes purified from healthy subjects with age ranging from the third to the tenth decade. We used linear regression analysis to determine if there is a linear relationship between age and cell frequencies. For the whole group, we find that with age there is a significant decline in the percentage of naïve T cells and CD8(+) T cells, and an increase in the percentage of effector memory cells, CD4(+)foxp3(+) T cells and NK cells. For all cells where there was an effect of ageing, the slope of the curve was greater for men than for women and this was statistically significant for CD8(+)alphabeta(+) T cells and CD3(+)CD45RA(-)CCR7(-) effector memory cells. There was also a difference for naïve cells but this was not significant.
The cause of the change in percentage of lymphocyte subsets with age, and the different effects on males and females is not fully understood but warrants further study.
据报道，随着年龄的增长，免疫功能会下降，循环淋巴细胞的频率也会发生改变。男性和女性的衰老和寿命也存在差异。我们进行这项研究是为了观察在不同年龄段的淋巴细胞亚群频率上，男性和女性是否存在差异。
我们使用流式细胞术检测了从第三到第十个十年龄段的健康受试者外周血白细胞中不同的群体。我们使用线性回归分析来确定年龄与细胞频率之间是否存在线性关系。对于整个组，我们发现随着年龄的增长，幼稚 T 细胞和 CD8(+) T 细胞的百分比显著下降，效应记忆细胞、CD4(+)foxp3(+) T 细胞和 NK 细胞的百分比增加。对于所有受衰老影响的细胞，男性的曲线斜率大于女性，这在 CD8(+)alphabeta(+) T 细胞和 CD3(+)CD45RA(-)CCR7(-)效应记忆细胞中具有统计学意义。幼稚细胞也存在差异，但没有统计学意义。
导致淋巴细胞亚群百分比随年龄变化的原因，以及对男性和女性的不同影响尚不完全清楚，但值得进一步研究。
