Tsukamura M
Department of Microbiology, Fujita Health University School of Medicine, Aichi, Japan.
Kekkaku. 1991 Feb;66(2):75-9.
In 0.1% Tween 80 aqueous solution or in 0.1% Tween 80-containing saline (0.9% NaCl aqueous solution), Mycobacterium smegmatis strain Jucho bacteria were alive for 3 days, whereas, in 0.1% Tween 80-containing phosphate buffer solution (pH 7.1), the bacteria died rapidly. Since it was shown previously that M. smegmatis is one of the mycobacteria most susceptible to Tween 80 (Tsukamura, M.: Kekkaku 63: 695-699, 1988), the 0.1% Tween 80 aqueous solution or 0.1% Tween 80-containing saline may be used for suspending mycobacteria in order to prevent the clumping. Incorporation of 32P-ortho-phosphate to the nucleic acid fraction was most markedly inhibited by the presence of 0.5% Tween 80 in 0.067 M phosphate buffer solution (pH 7.1). The finding shows that Tween 80 inhibits directly or indirectly the synthesis of nucleic acids. Tween 80 was not bactericidal in an aqueous solution but acted bactericidally in the co-existence of phosphate, and such bactericidal activity was completely diminished by adding an ammoniacal nitrogen compound (Tsukamura, M.: Medicine and Biology (Tokyo) 96: 159-161, 1978). These findings suggest that Tween 80 does not simply act as a detergent but interferes with some metabolic way in the synthesis of nucleic acids.
耻垢分枝杆菌菌株寿超菌在0.1%吐温80水溶液或含0.1%吐温80的生理盐水（0.9%氯化钠水溶液）中可存活3天，而在含0.1%吐温80的磷酸盐缓冲溶液（pH 7.1）中，细菌迅速死亡。由于先前已表明耻垢分枝杆菌是对吐温80最敏感的分枝杆菌之一（冢村，M.：《结核》63：695 - 699，1988），0.1%吐温80水溶液或含0.1%吐温80的生理盐水可用于悬浮分枝杆菌以防止结块。在0.067 M磷酸盐缓冲溶液（pH 7.1）中存在0.5%吐温80时，核酸部分对32P - 正磷酸盐的掺入受到最显著抑制。这一发现表明吐温80直接或间接抑制核酸的合成。吐温80在水溶液中无杀菌作用，但在磷酸盐共存时具有杀菌作用，并且通过添加氨氮化合物这种杀菌活性完全消失（冢村，M.：《医学与生物学》（东京）96：159 - 161，1978）。这些发现表明吐温80并非仅仅作为一种洗涤剂起作用，而是在核酸合成中干扰了某些代谢途径。
