Laboratory of Entomology, Wageningen University, Wageningen, The Netherlands.
Insect Mol Biol. 2010 Jun 1;19(3):367-79. doi: 10.1111/j.1365-2583.2010.00997.x. Epub 2010 Mar 4.
The cAMP/PKA signalling pathway and transcription factor cAMP response element-binding protein (CREB) play key roles in long-term memory (LTM) formation. We used two closely related parasitic wasp species, Cotesia glomerata and Cotesia rubecula, which were previously shown to be different in LTM formation, and sequenced at least nine different CREB transcripts in both wasp species. The splicing patterns, functional domains and amino acid sequences were similar to those found in the CREB genes of other organisms. The predicted amino acid sequences of the CREB isoforms were identical in both wasp species. Using real-time quantitative PCR we found that two low abundant CREB transcripts are differentially expressed in the two wasps, whereas the expression levels of high abundant transcripts are similar.
cAMP/PKA 信号通路和转录因子 cAMP 反应元件结合蛋白 (CREB) 在长时记忆 (LTM) 形成中发挥着关键作用。我们使用了两种密切相关的寄生蜂物种，即甘蓝夜蛾绒茧蜂和微红绒茧蜂，它们之前在 LTM 形成方面表现出不同，并在这两种蜂中至少测序了九个不同的 CREB 转录本。剪接模式、功能域和氨基酸序列与其他生物体的 CREB 基因相似。两种蜂的 CREB 同工型的预测氨基酸序列相同。通过实时定量 PCR，我们发现两种低丰度的 CREB 转录本在两种蜂中存在差异表达，而高丰度转录本的表达水平相似。
