Falzone N, Huyser C, Becker P, Leszczynski D, Franken D R
Department of Biomedical Sciences, Tshwane University of Technology, Pretoria, South Africa.
Int J Androl. 2011 Feb;34(1):20-6. doi: 10.1111/j.1365-2605.2010.01054.x.
Several recent studies have indicated that radiofrequency electromagnetic fields (RF-EMF) have an adverse effect on human sperm quality, which could translate into an effect on fertilization potential. This study evaluated the effect of RF-EMF on sperm-specific characteristics to assess the fertilizing competence of sperm. Highly motile human spermatozoa were exposed for 1 h to 900-MHz mobile phone radiation at a specific absorption rate of 2.0 W/kg and examined at various times after exposure. The acrosome reaction was evaluated using flow cytometry. The radiation did not affect sperm propensity for the acrosome reaction. Morphometric parameters were assessed using computer-assisted sperm analysis. Significant reduction in sperm head area (9.2 ± 0.7 μm² vs. 18.8 ± 1.4 μm²) and acrosome percentage of the head area (21.5 ± 4% vs. 35.5 ± 11.4%) was reported among exposed sperm compared with unexposed controls. Sperm-zona binding was assessed directly after exposure using the hemizona assay. The mean number of zona-bound sperm of the test hemizona and controls was 22.8 ± 12.4 and 31.8 ± 12.8 (p < 0.05), respectively. This study concludes that although RF-EMF exposure did not adversely affect the acrosome reaction, it had a significant effect on sperm morphometry. In addition, a significant decrease in sperm binding to the hemizona was observed. These results could indicate a significant effect of RF-EMF on sperm fertilization potential.
最近的几项研究表明，射频电磁场（RF-EMF）对人类精子质量有不利影响，这可能转化为对受精潜能的影响。本研究评估了RF-EMF对精子特定特征的影响，以评估精子的受精能力。将高活力的人类精子以2.0 W/kg的比吸收率暴露于900 MHz的手机辐射1小时，并在暴露后的不同时间进行检查。使用流式细胞术评估顶体反应。辐射不影响精子顶体反应的倾向。使用计算机辅助精子分析评估形态学参数。与未暴露的对照组相比，暴露精子的精子头部面积（9.2±0.7μm²对18.8±1.4μm²）和头部顶体百分比（21.5±4%对35.5±11.4%）显著降低。暴露后立即使用半透明带试验评估精子-透明带结合。试验半透明带和对照组的透明带结合精子平均数分别为22.8±12.4和31.8±12.8（p<0.05）。本研究得出结论，虽然暴露于RF-EMF对顶体反应没有不利影响，但对精子形态学有显著影响。此外，观察到精子与半透明带的结合显著减少。这些结果可能表明RF-EMF对精子受精潜能有显著影响。
