Person P, Papierman S, Zipper H, Libbin R M
Calcif Tissue Res. 1977 Dec 14;24(1):37-9. doi: 10.1007/BF02223294.
This study attempted to detect evidence of mitochondrial terminal respiratory components in matrix vesicles isolated from rachitic rat tibial epiphyseal plates. Biochemical assays for cytochrome c oxidase, NAD isocitrate dehydrogenase, NADP isocitrate dehydrogenase and succinate-cytochrome c reductase were negative. Polarimetric determinations revealed that the addition of succinate to matrix vesicles in suspension did not cause any increase in oxygen utilization. Spectrophotometric tracings of deoxycholate-solubilized matrix vesicles showed no characteristic absorption peaks or maxima belonging to any of the cytochrome complex components. Attempts to prepare pyridine hemochromes of cytochrome prosthetic groups from the matrix vesicles were also unsuccessful. The above results indicate that key components of mitochondrial respiratory systems are not detectable in rachitic matrix vesicles. The results are compatible with the interpretation that such vesicles are not derived from mitochondria.
本研究试图在从佝偻病大鼠胫骨骨骺板分离出的基质小泡中检测线粒体末端呼吸成分的证据。细胞色素c氧化酶、NAD异柠檬酸脱氢酶、NADP异柠檬酸脱氢酶和琥珀酸 - 细胞色素c还原酶的生化测定均为阴性。偏振测定表明，向悬浮的基质小泡中添加琥珀酸不会导致氧利用率增加。脱氧胆酸盐增溶的基质小泡的分光光度描记图未显示属于任何细胞色素复合体成分的特征吸收峰或最大值。从基质小泡制备细胞色素辅基的吡啶血色素的尝试也未成功。上述结果表明，在佝偻病基质小泡中无法检测到线粒体呼吸系统的关键成分。这些结果与这样的解释相符，即此类小泡并非源自线粒体。
