Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
Mol Biol Rep. 2011 Jan;38(1):17-21. doi: 10.1007/s11033-010-0072-8. Epub 2010 Mar 18.
To identify a predictor to forecast superovulation response on the basis of associations between superovulation performance and gene polymorphism, the PCR-RFLP method was applied to detect an A>G transition determining an MspI polymorphism at position 192 in the exon I of the bovine inhibin alpha (INHA) gene and evaluate its associations with superovulatory response in 118 Chinese Holstein cows treated for superovulation. Association analysis showed that cows with the GG genotype resulted in a significant increase in the number of ova (TNO) than AG and AA genotypes in the first (P=0.023), second (P=0.004) and third (P=0.002) superovulation treatments and produced more transferable embryos (NTE) than that of AG and AA genotypes in the third (P=0.045) superovulation treatment. Moreover, individuals with GG genotype produced more transferable embryos than AA (P<0.05) genotype in the second superovulation treatment and all cows without superovulation response were mutations with genotypes of AA and AG. These results indicate that INHA gene can be used as a predictor for superovulation in Chinese Holstein cows, and imply that cows with AA genotype should be excluded for superovulation practices.
为了基于超数排卵性能与基因多态性之间的关联来鉴定预测超数排卵反应的指标，应用 PCR-RFLP 方法检测牛抑制素α（INHA）基因外显子 I 第 192 位决定 MspI 多态性的 A>G 转换，并评估其与 118 头中国荷斯坦奶牛超数排卵反应的关系。关联分析表明，与 AG 和 AA 基因型相比，GG 基因型的奶牛在第一次（P=0.023）、第二次（P=0.004）和第三次（P=0.002）超数排卵处理中获得的卵母细胞数（TNO）显著增加，并且在第三次（P=0.045）超数排卵处理中获得的可移植胚胎数（NTE）多于 AG 和 AA 基因型。此外，与 AA 基因型相比，GG 基因型的个体在第二次超数排卵处理中产生了更多的可移植胚胎（P<0.05），并且所有没有超数排卵反应的个体都是 AA 和 AG 基因型的突变体。这些结果表明，INHA 基因可以作为中国荷斯坦奶牛超数排卵的预测指标，并且暗示 AA 基因型的奶牛应该被排除在超数排卵实践之外。
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