Nikitkina Elena, Krutikova Anna, Musidray Artem, Plemyashov Kirill
Russian Research Institute of Farm Animal Genetics and Breeding-Branch of the L.K. Ernst Federal Research Center for Animal Husbandry, 55A, Moskovskoye sh., 196625 Saint-Petersburg, Russia.
Animals (Basel). 2021 Oct 2;11(10):2882. doi: 10.3390/ani11102882.
The aim of the study was to search for new mutations in the previously studied gene loci of follicle-stimulating hormone receptor (), inhibin α (), inhibin β A (), prolactin (), transition protein 2 (), and sperm flagella 2 () by sequencing, as well as the search for associations of previously identified mutations at these loci with fresh semen quality in Russian Holstein bulls. Phenotypic data from 189 bulls was collected. Data was analyzed for most bulls for three years of semen collection. The maximum value of each semen quality indicator (doublet ejaculate volume, sperm concentration, progressive motility and total number of spermatozoa) were selected. SNPs were identified in the , , , , genes. The gene did not have polymorphism. Significant ( < 0.05) associations of polymorphisms in the gene with double ejaculate volume, concentration and total number of spermatozoa were identified. Polymorphism in the gene was significantly associated ( < 0.05) with sperm concentration. Polymorphism in the gene was significantly associated ( < 0.05) with doublet ejaculate volume and total number of spermatozoa. Polymorphisms in the and genes did not have significant associations with semen quality. The SNPs studied in our pilot work may be considered as candidate genetic markers in the selection of bulls.
本研究的目的是通过测序在先前研究的促卵泡激素受体（）、抑制素α（）、抑制素βA（）、催乳素（）、过渡蛋白2（）和精子鞭毛2（）基因位点中寻找新的突变，以及寻找这些位点上先前鉴定的突变与俄罗斯荷斯坦公牛新鲜精液质量之间的关联。收集了189头公牛的表型数据。对大多数公牛三年的精液采集数据进行了分析。选取了每个精液质量指标（双份射精量、精子浓度、前向运动率和精子总数）的最大值。在、、、、基因中鉴定出单核苷酸多态性（SNP）。基因没有多态性。鉴定出基因中的多态性与双份射精量、精子浓度和精子总数之间存在显著（<0.05）关联。基因中的多态性与精子浓度显著相关（<0.05）。基因中的多态性与双份射精量和精子总数显著相关（<0.05）。和基因中的多态性与精液质量没有显著关联。我们初步研究中所研究的SNP可被视为公牛选择中的候选遗传标记。
