Analysis of Expression and Single Nucleotide Polymorphisms of Gene Associated with Reproductive Traits in Chickens.

Inhibin () is a candidate gene controlling ovulation in poultry. As the functional center of inhibin, is a molecular marker associated with egg-laying performance. The objective of the current study was to analyze the expression differences of in reproductive system and single nucleotide polymorphisms (SNPs) associations with reproductive traits in chickens. A total of 260 LuHua chickens (barred-feather chicken) were adopted. Twelve SNPs were detected in gene. Among the exonic SNPs, three (g. 22177991A>G, g. 22178249G>C, and g. 22178414G>A) were missense mutations, resulting in the amino acid substitutions Val→Ala, Ala→Gly, and Ala→Gly, respectively. Four SNPs in the 3＇ untranslated region of were predicted to either disturb or create microRNA-target interactions. Five SNPs (g. 22176870T>C, g. 22177100T>C, g. 22177149T>C, g. 22177991A>G, and g. 22178975G>A) were significantly associated with the number of eggs at 300 d of age (EN) ( < 0.05). Birds carrying GA genotype exhibited more EN than those with AA genotype ( < 0.01). In addition, quantitative real-time PCR revealed that is mainly expressed in follicles on d 300 in chickens. Firstly, expression increased and then decreased. The highest mRNA abundance was found in the fifth largest preovulatory follicle (F5) ( < 0.01). In the prehierarchical follicles, mRNA expression increased dramatically in small yellow follicles (SYF) ( < 0.01). Western blotting analysis showed that the INHA protein expression profile in the follicle was similar to its mRNA counterpart with greater expression in F5 and SYF follicles and lowest expression in F1 follicles ( < 0.05). These results suggest that is a potential candidate gene improving reproductive traits in chickens.