Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.
Biomed Res Int. 2019 Aug 8;2019:8572837. doi: 10.1155/2019/8572837. eCollection 2019.
Inhibin () is a candidate gene controlling ovulation in poultry. As the functional center of inhibin, is a molecular marker associated with egg-laying performance. The objective of the current study was to analyze the expression differences of in reproductive system and single nucleotide polymorphisms (SNPs) associations with reproductive traits in chickens. A total of 260 LuHua chickens (barred-feather chicken) were adopted. Twelve SNPs were detected in gene. Among the exonic SNPs, three (g. 22177991A>G, g. 22178249G>C, and g. 22178414G>A) were missense mutations, resulting in the amino acid substitutions Val→Ala, Ala→Gly, and Ala→Gly, respectively. Four SNPs in the 3＇ untranslated region of were predicted to either disturb or create microRNA-target interactions. Five SNPs (g. 22176870T>C, g. 22177100T>C, g. 22177149T>C, g. 22177991A>G, and g. 22178975G>A) were significantly associated with the number of eggs at 300 d of age (EN) ( < 0.05). Birds carrying GA genotype exhibited more EN than those with AA genotype ( < 0.01). In addition, quantitative real-time PCR revealed that is mainly expressed in follicles on d 300 in chickens. Firstly, expression increased and then decreased. The highest mRNA abundance was found in the fifth largest preovulatory follicle (F5) ( < 0.01). In the prehierarchical follicles, mRNA expression increased dramatically in small yellow follicles (SYF) ( < 0.01). Western blotting analysis showed that the INHA protein expression profile in the follicle was similar to its mRNA counterpart with greater expression in F5 and SYF follicles and lowest expression in F1 follicles ( < 0.05). These results suggest that is a potential candidate gene improving reproductive traits in chickens.
抑制素（）是控制家禽排卵的候选基因。作为抑制素的功能中心，是与产蛋性能相关的分子标记。本研究旨在分析鸡生殖系统中基因的表达差异及与繁殖性状相关的单核苷酸多态性（SNP）。采用 260 只芦花鸡（带羽鸡）。在基因中检测到 12 个 SNP。在外显子 SNP 中，有 3 个（g.22177991A>G、g.22178249G>C 和 g.22178414G>A）为错义突变，导致相应的氨基酸取代分别为缬氨酸→丙氨酸、丙氨酸→甘氨酸和丙氨酸→甘氨酸。基因 3'非翻译区的 4 个 SNP 预测会干扰或创建 microRNA 靶相互作用。5 个 SNP（g.22176870T>C、g.22177100T>C、g.22177149T>C、g.22177991A>G 和 g.22178975G>A）与 300 日龄蛋数（EN）显著相关（<0.05）。携带 GA 基因型的鸡比携带 AA 基因型的鸡具有更多的 EN（<0.01）。此外，实时定量 PCR 显示，在鸡中，基因主要在 300 日龄时的卵泡中表达。首先，表达增加，然后减少。第五大预排卵卵泡（F5）中的 mRNA 丰度最高（<0.01）。在预层级卵泡中，小黄体卵泡（SYF）中的 mRNA 表达显著增加（<0.01）。Western blot 分析显示，卵泡中 INHA 蛋白表达谱与其 mRNA 相似，F5 和 SYF 卵泡中表达较高，F1 卵泡中表达较低（<0.05）。这些结果表明，是提高鸡繁殖性状的潜在候选基因。
