Daskal Y, Woodard C, Crooke S T, Busch H
Cancer Res. 1978 Feb;38(2):467-73.
This study was designed to determine the effects of several antitimor anthracyclines, including Adriamycin and its analogs, carminomycin and marcellomycin, on the ultrastructure of nucleoli of Novikoff hepatoma cells. Adriamycin and carminomycin, which are structurally related, induce nucleolar segregation following the formation of conspicuous fibrillar centers. Marcellomycin did not induce formation of nucleolar fibrillar centers. Instead, numerous microspherules formed following treatment with marcellomycin; later complete nucleolar segregation developed. The microspherules were observed to be in various stages of extrusion from the nucleolar body. This microspherule "migration" appeared to be both time and drug concentration dependent. These results show that the rate and extent of nucleolar ultrastructural aberration may be related to structural differences of the various anthracyclines.
本研究旨在确定几种抗肿瘤蒽环类药物，包括阿霉素及其类似物、洋红霉素和马塞洛霉素，对诺维科夫肝癌细胞核仁超微结构的影响。结构相关的阿霉素和洋红霉素在形成明显的纤维中心后诱导核仁分离。马塞洛霉素未诱导核仁纤维中心的形成。相反，用马塞洛霉素处理后形成了大量微球；随后出现了完全的核仁分离。观察到微球处于从核仁体挤出的不同阶段。这种微球“迁移”似乎与时间和药物浓度有关。这些结果表明，核仁超微结构异常的速率和程度可能与各种蒽环类药物的结构差异有关。
