尿纤溶酶原激活物生长因子结构域中翻译后岩藻糖基化的特征分析。
Characterization of a posttranslational fucosylation in the growth factor domain of urinary plasminogen activator.
作者信息
Buko A M, Kentzer E J, Petros A, Menon G, Zuiderweg E R, Sarin V K
机构信息
Analytical Research Department, Abbott Laboratories, North Chicago, IL 60064.
出版信息
Proc Natl Acad Sci U S A. 1991 May 1;88(9):3992-6. doi: 10.1073/pnas.88.9.3992.
Abstract
A posttranslational modification site in natural and recombinant urinary-type plasminogen activators (urokinases; EC 3.4.21.31) has been localized to Thr-18, in the growth factor domain of the molecule. This is the region of urinary plasminogen activator responsible for its specific receptor binding. An unusual carbohydrate-protein linkage, a single monosaccharide, fucose, covalently attached directly to threonine in the peptide, is described here. The glycan moiety and the site of modification have been identified with mass spectrometry and confirmed by carbohydrate composition analysis, Edman degradation, and one- and two-dimensional NMR studies. This type of modification is normally not detected without mass spectrometry because the fucose-threonine bond is hydrolyzed under standard acidic conditions of the amino acid analysis and Edman sequencing. This modification may be widely found in other proteins.
摘要
天然和重组尿激酶型纤溶酶原激活剂（尿激酶；EC 3.4.21.31）的一个翻译后修饰位点已定位到分子生长因子结构域中的苏氨酸-18。这是尿纤溶酶原激活剂负责其特异性受体结合的区域。本文描述了一种不寻常的碳水化合物-蛋白质连接方式，即单个单糖岩藻糖直接共价连接到肽中的苏氨酸上。聚糖部分和修饰位点已通过质谱鉴定，并通过碳水化合物组成分析、埃德曼降解以及一维和二维核磁共振研究得到证实。如果没有质谱分析，这种修饰通常无法检测到，因为在氨基酸分析和埃德曼测序的标准酸性条件下，岩藻糖-苏氨酸键会被水解。这种修饰可能在其他蛋白质中广泛存在。
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