Guilford P J, Ziegler-Graff V, Baulcombe D C
Department of Molecular Genetics, Institute of Plant Science Research, Cambridge, United Kingdom.
Virology. 1991 Jun;182(2):607-14. doi: 10.1016/0042-6822(91)90601-7.
The function of the 16-kDa protein encoded by tobacco rattle virus (TRV) RNA-1 was investigated by a mutational analysis of the 16-kDa protein gene. Transcripts of TRV RNA-1 produced from a full-length cDNA clone of TRV RNA-1 (SYM strain) remained infectious when the 16-kDa protein gene was disrupted by premature termination codons and a deletion which removed 73% of the coding region. A deletion which included the intergenic region between the 29-kDa protein gene and the 16-kDa protein gene, the entire 16-kDa protein coding region, and 57% of the 3' noncoding region was not infectious. Transcripts in which the 16-kDa protein coding region was replaced by the tobacco mosaic virus (TMV) (L strain) coat protein gene were also infectious and expressed TMV coat protein in infected tissue. Inclusion of the TMV origin of assembly sequence in the chimaeric constructs resulted in the accumulation of TMV-like virus particles in infected tissue.
通过对烟草脆裂病毒（TRV）RNA - 1编码的16 kDa蛋白基因进行突变分析，研究了该蛋白的功能。当16 kDa蛋白基因被提前终止密码子和一个缺失了73%编码区的缺失突变破坏时，由TRV RNA - 1（SYM株系）全长cDNA克隆产生的TRV RNA - 1转录本仍具有感染性。一个缺失突变，包括29 kDa蛋白基因和16 kDa蛋白基因之间的基因间隔区、整个16 kDa蛋白编码区以及3'非编码区的57%，是没有感染性的。将16 kDa蛋白编码区替换为烟草花叶病毒（TMV）（L株系）外壳蛋白基因的转录本也具有感染性，并在受感染组织中表达了TMV外壳蛋白。在嵌合构建体中包含TMV组装起始序列导致在受感染组织中积累了类似TMV的病毒颗粒。
