烟草脆裂病毒TCM株系的RNA 2编码一个意外基因。
RNA 2 of tobacco rattle virus strain TCM encodes an unexpected gene.
作者信息
Angenent G C, Linthorst H J, van Belkum A F, Cornelissen B J, Bol J F
出版信息
Nucleic Acids Res. 1986 Jun 11;14(11):4673-82. doi: 10.1093/nar/14.11.4673.
Abstract
The sequence of the 3'-terminal 1210 nucleotides of RNA 1 and the complete sequence of 3389 nucleotides of RNA 2 of tobacco rattle virus (TRV) strain TCM has been deduced. The sequence of the 3'-terminal 1099 nucleotides of RNAs 1 and 2 was found to be identical. Thus the genome of this TRV strain is partially diploid, encoding a 16K protein in both RNA 1 and RNA 2. The sequence that is unique to RNA 2 contains two open reading frames: the coat protein cistron and a cistron for a 29.1K protein, which shows no homology with the RNA 1 encoded 28.8K protein. cDNA probes corresponding to these two open reading frames cross-hybridized to pea early-browning virus RNA 2, but not to RNA 2 of five other tobraviruses tested.
摘要
已推导得出烟草脆裂病毒（TRV）TCM株系RNA 1的3'末端1210个核苷酸序列以及RNA 2的3389个核苷酸的完整序列。发现RNA 1和RNA 2的3'末端1099个核苷酸序列相同。因此，该TRV株系的基因组是部分二倍体，在RNA 1和RNA 2中均编码一种16K蛋白。RNA 2特有的序列包含两个开放阅读框：外壳蛋白顺反子和一个编码29.1K蛋白的顺反子，该蛋白与RNA 1编码的28.8K蛋白无同源性。与这两个开放阅读框对应的cDNA探针与豌豆早褐病毒RNA 2交叉杂交，但与所测试的其他五种烟草病毒的RNA 2不杂交。
相似文献
1
RNA 2 of tobacco rattle virus strain TCM encodes an unexpected gene.烟草脆裂病毒TCM株系的RNA 2编码一个意外基因。
Nucleic Acids Res. 1986 Jun 11;14(11):4673-82. doi: 10.1093/nar/14.11.4673.
2
Analysis of the genome structure of tobacco rattle virus strain PSG.烟草脆裂病毒PSG株系的基因组结构分析
Nucleic Acids Res. 1986 Mar 11;14(5):2157-69. doi: 10.1093/nar/14.5.2157.
3
Nucleotide sequence of both genomic RNAs of a North American tobacco rattle virus isolate.一种北美烟草脆裂病毒分离株的两个基因组RNA的核苷酸序列。
Arch Virol. 1998;143(8):1535-44. doi: 10.1007/s007050050396.
4
Expression of the 16K cistron of tobacco rattle virus in protoplasts.烟草脆裂病毒16K顺反子在原生质体中的表达。
Virology. 1989 Apr;169(2):305-11. doi: 10.1016/0042-6822(89)90155-4.
5
Genome structure of tobacco rattle virus strain PLB: further evidence on the occurrence of RNA recombination among tobraviruses.烟草脆裂病毒PLB株系的基因组结构：烟草病毒属病毒间存在RNA重组的进一步证据
Virology. 1989 Jul;171(1):271-4. doi: 10.1016/0042-6822(89)90537-0.
6
A model for the generation of tobacco rattle virus (TRV) anomalous isolates: pea early browning virus RNA-2 acquires TRV sequences from both RNA-1 and RNA-2.烟草脆裂病毒（TRV）异常分离株产生的模型：豌豆早褐病毒RNA - 2从RNA - 1和RNA - 2获取TRV序列。
J Gen Virol. 1991 Jul;72 ( Pt 7):1751-4. doi: 10.1099/0022-1317-72-7-1751.
7
Transgenic tobacco plants accumulating tobacco rattle virus coat protein resist infection with tobacco rattle virus and pea early browning virus.积累烟草脆裂病毒外壳蛋白的转基因烟草植株能抵抗烟草脆裂病毒和豌豆早褐病毒的感染。
Virology. 1988 Dec;167(2):649-52. doi: 10.1016/s0042-6822(88)90131-6.
8
Susceptibility to virus infection of transgenic tobacco plants expressing structural and nonstructural genes of tobacco rattle virus.表达烟草脆裂病毒结构和非结构基因的转基因烟草植株对病毒感染的易感性。
Virology. 1990 Mar;175(1):191-8. doi: 10.1016/0042-6822(90)90199-2.
9
Complete genomic sequence of a Tobacco rattle virus isolate from Michigan-grown potatoes.密歇根州种植的马铃薯中分离的烟草脆裂病毒分离物的完整基因组序列。
Arch Virol. 2010 Apr;155(4):621-5. doi: 10.1007/s00705-010-0609-0. Epub 2010 Mar 11.
10
The nucleotide sequence of tobacco rattle virus RNA-2 (CAM strain).烟草脆裂病毒RNA-2（CAM株系）的核苷酸序列
Nucleic Acids Res. 1985 Dec 9;13(23):8507-18. doi: 10.1093/nar/13.23.8507.
引用本文的文献
1
Prevention and treatment of COVID-19 using Traditional Chinese Medicine: A review.运用中医药防治 COVID-19：综述。
Phytomedicine. 2021 May;85:153308. doi: 10.1016/j.phymed.2020.153308. Epub 2020 Aug 20.
2
Unique RNA 2 sequences of two Brazilian isolates of Pepper ringspot virus, a tobravirus.辣椒环斑病毒（烟草脆裂病毒属）的两个巴西分离株的独特RNA 2序列。
Virus Genes. 2014 Aug;49(1):169-73. doi: 10.1007/s11262-014-1066-8. Epub 2014 Apr 23.
3
Heterologous virus-induced gene silencing as a promising approach in plant functional genomics.异源病毒诱导的基因沉默作为一种很有前途的植物功能基因组学研究方法。
Mol Biol Rep. 2012 Mar;39(3):2169-78. doi: 10.1007/s11033-011-0965-1. Epub 2011 Jun 8.
4
Gene expression from viral RNA genomes.来自病毒RNA基因组的基因表达。
Plant Mol Biol. 1996 Oct;32(1-2):367-91. doi: 10.1007/BF00039391.
5
Serial passage of tobacco rattle virus under different selection conditions results in deletion of structural and nonstructural genes in RNA 2.烟草脆裂病毒在不同选择条件下的连续传代导致RNA 2中结构基因和非结构基因的缺失。
J Virol. 1996 Aug;70(8):4933-40. doi: 10.1128/JVI.70.8.4933-4940.1996.
6
Long, nearly identical untranslated sequences at the 3' terminal regions of the genomic RNAs of cherry leafroll virus (walnut strain).樱桃叶卷病毒（核桃株系）基因组RNA 3'末端区域存在长的、几乎相同的未翻译序列。
Virus Genes. 1995;10(3):245-52. doi: 10.1007/BF01701814.
7
A phylogenetically conserved sequence within viral 3' untranslated RNA pseudoknots regulates translation.病毒3'非翻译RNA假结内的一个系统发育保守序列调控翻译。
Mol Cell Biol. 1993 Sep;13(9):5331-47. doi: 10.1128/mcb.13.9.5331-5347.1993.
8
tRNA-like properties of tobacco rattle virus RNA.烟草脆裂病毒RNA的类tRNA特性
Nucleic Acids Res. 1987 Apr 10;15(7):2837-50. doi: 10.1093/nar/15.7.2837.
9
A new 'sense' RNA approach to block viral RNA replication in vitro.一种在体外阻断病毒RNA复制的新型“传感”RNA方法。
Nucleic Acids Res. 1987 May 26;15(10):4123-30. doi: 10.1093/nar/15.10.4123.
10
Sequence and organization of barley yellow dwarf virus genomic RNA.大麦黄矮病毒基因组RNA的序列与结构
Nucleic Acids Res. 1988 Jul 11;16(13):6097-111. doi: 10.1093/nar/16.13.6097.
本文引用的文献
1
The organisation and interviral homologies of genes at the 3' end of tobacco rattle virus RNA1.烟草脆裂病毒 RNA1 3' 端基因的组织和间隔同源性。
EMBO J. 1986 Feb;5(2):223-9. doi: 10.1002/j.1460-2075.1986.tb04202.x.
2
Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.缓冲液梯度凝胶和35S标记辅助快速DNA序列测定。
Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5. doi: 10.1073/pnas.80.13.3963.
3
New versatile cloning and sequencing vectors based on bacteriophage M13.基于噬菌体M13的新型通用克隆和测序载体。
Gene. 1983 Dec;26(1):91-9. doi: 10.1016/0378-1119(83)90039-2.
4
New M13 vectors for cloning.用于克隆的新型M13载体。
Methods Enzymol. 1983;101:20-78. doi: 10.1016/0076-6879(83)01005-8.
5
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.用核酸外切酶III进行单向消化可为DNA测序创建靶向断点。
Gene. 1984 Jun;28(3):351-9. doi: 10.1016/0378-1119(84)90153-7.
6
Chemical probes for higher-order structure in RNA.用于研究RNA高阶结构的化学探针。
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4679-82. doi: 10.1073/pnas.77.8.4679.
7
Analysis of the genome structure of tobacco rattle virus strain PSG.烟草脆裂病毒PSG株系的基因组结构分析
Nucleic Acids Res. 1986 Mar 11;14(5):2157-69. doi: 10.1093/nar/14.5.2157.
8
The nucleotide sequence of tobacco rattle virus RNA-2 (CAM strain).烟草脆裂病毒RNA-2（CAM株系）的核苷酸序列
Nucleic Acids Res. 1985 Dec 9;13(23):8507-18. doi: 10.1093/nar/13.23.8507.
9
Translation of tobacco rattle virus RNA in vitro using wheat germ extracts.利用小麦胚提取物在体外翻译烟草脆裂病毒RNA
Virology. 1976 Feb;69(2):408-15. doi: 10.1016/0042-6822(76)90472-4.
10
Studies on sequence homology between the RNA's of alfalfa mosaic virus.苜蓿花叶病毒RNA之间的序列同源性研究。
Virology. 1975 May;65(1):1-15. doi: 10.1016/0042-6822(75)90002-1.
Zotero 插件