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RNA 2 of tobacco rattle virus strain TCM encodes an unexpected gene.烟草脆裂病毒TCM株系的RNA 2编码一个意外基因。
Nucleic Acids Res. 1986 Jun 11;14(11):4673-82. doi: 10.1093/nar/14.11.4673.
2
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本文引用的文献

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The organisation and interviral homologies of genes at the 3' end of tobacco rattle virus RNA1.烟草脆裂病毒 RNA1 3' 端基因的组织和间隔同源性。
EMBO J. 1986 Feb;5(2):223-9. doi: 10.1002/j.1460-2075.1986.tb04202.x.
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Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.缓冲液梯度凝胶和35S标记辅助快速DNA序列测定。
Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5. doi: 10.1073/pnas.80.13.3963.
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New versatile cloning and sequencing vectors based on bacteriophage M13.基于噬菌体M13的新型通用克隆和测序载体。
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New M13 vectors for cloning.用于克隆的新型M13载体。
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Chemical probes for higher-order structure in RNA.用于研究RNA高阶结构的化学探针。
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7
Analysis of the genome structure of tobacco rattle virus strain PSG.烟草脆裂病毒PSG株系的基因组结构分析
Nucleic Acids Res. 1986 Mar 11;14(5):2157-69. doi: 10.1093/nar/14.5.2157.
8
The nucleotide sequence of tobacco rattle virus RNA-2 (CAM strain).烟草脆裂病毒RNA-2（CAM株系）的核苷酸序列
Nucleic Acids Res. 1985 Dec 9;13(23):8507-18. doi: 10.1093/nar/13.23.8507.
9
Translation of tobacco rattle virus RNA in vitro using wheat germ extracts.利用小麦胚提取物在体外翻译烟草脆裂病毒RNA
Virology. 1976 Feb;69(2):408-15. doi: 10.1016/0042-6822(76)90472-4.
10
Studies on sequence homology between the RNA's of alfalfa mosaic virus.苜蓿花叶病毒RNA之间的序列同源性研究。
Virology. 1975 May;65(1):1-15. doi: 10.1016/0042-6822(75)90002-1.

烟草脆裂病毒TCM株系的RNA 2编码一个意外基因。

RNA 2 of tobacco rattle virus strain TCM encodes an unexpected gene.

作者信息

Angenent G C, Linthorst H J, van Belkum A F, Cornelissen B J, Bol J F

出版信息

Nucleic Acids Res. 1986 Jun 11;14(11):4673-82. doi: 10.1093/nar/14.11.4673.

DOI:10.1093/nar/14.11.4673
PMID:3714489
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC311473/
Abstract

The sequence of the 3'-terminal 1210 nucleotides of RNA 1 and the complete sequence of 3389 nucleotides of RNA 2 of tobacco rattle virus (TRV) strain TCM has been deduced. The sequence of the 3'-terminal 1099 nucleotides of RNAs 1 and 2 was found to be identical. Thus the genome of this TRV strain is partially diploid, encoding a 16K protein in both RNA 1 and RNA 2. The sequence that is unique to RNA 2 contains two open reading frames: the coat protein cistron and a cistron for a 29.1K protein, which shows no homology with the RNA 1 encoded 28.8K protein. cDNA probes corresponding to these two open reading frames cross-hybridized to pea early-browning virus RNA 2, but not to RNA 2 of five other tobraviruses tested.

摘要

已推导得出烟草脆裂病毒（TRV）TCM株系RNA 1的3'末端1210个核苷酸序列以及RNA 2的3389个核苷酸的完整序列。发现RNA 1和RNA 2的3'末端1099个核苷酸序列相同。因此，该TRV株系的基因组是部分二倍体，在RNA 1和RNA 2中均编码一种16K蛋白。RNA 2特有的序列包含两个开放阅读框：外壳蛋白顺反子和一个编码29.1K蛋白的顺反子，该蛋白与RNA 1编码的28.8K蛋白无同源性。与这两个开放阅读框对应的cDNA探针与豌豆早褐病毒RNA 2交叉杂交，但与所测试的其他五种烟草病毒的RNA 2不杂交。