Hatake K, Wakabayashi I, Kakishita E, Taniguchi T, Ouchi H, Hishida S
Department of Legal Medicine, Hyogo College of Medicine, Japan.
Alcohol Clin Exp Res. 1991 Feb;15(1):112-5. doi: 10.1111/j.1530-0277.1991.tb00527.x.
Rats were maintained on liquid diets containing ethanol (35% of total calories) or an equicaloric volume of sucrose instead of ethanol for 10 wk. Vascular strips of isolated rat aortas were mounted in organ chambers to record isometric tension. Ethanol in vitro inhibited the endothelium-dependent relaxation responses to acetylcholine and ATP in both pair-fed control and ethanol-fed rats. The inhibitory effect of ethanol was greater in the pair-fed rats. In addition, the magnitudes of these relaxation responses in the absence of ethanol in vitro in pair-fed rats were similar to those in the presence of ethanol in ethanol-fed rats. In the absence of ethanol in vitro, the relaxations in response to acetylcholine and ATP in the ethanol-fed rats were greater than in the pair-fed rats. These results suggest that chronic ethanol consumption can induce tolerance to ethanol-induced inhibition of endothelium-dependent relaxation responses to acetylcholine and ATP, and that the relaxations can become adapted to the presence of plasma levels of ethanol, which may inhibit the relaxation in vivo. The augmented relaxation in the ethanol-fed rats may result from the mechanism causing tolerance to the inhibitory effect of ethanol.
将大鼠分别给予含乙醇（占总热量的35%）的液体饮食或等热量的蔗糖而非乙醇，持续10周。将分离的大鼠主动脉血管条安装在器官浴槽中记录等长张力。体外实验中，乙醇抑制了配对喂养对照组和乙醇喂养组大鼠对乙酰胆碱和ATP的内皮依赖性舒张反应。乙醇对配对喂养大鼠的抑制作用更强。此外，配对喂养大鼠在体外无乙醇时这些舒张反应的幅度与乙醇喂养大鼠在有乙醇时的幅度相似。在体外无乙醇时，乙醇喂养大鼠对乙酰胆碱和ATP的舒张反应大于配对喂养大鼠。这些结果表明，长期摄入乙醇可诱导对乙醇诱导的乙酰胆碱和ATP内皮依赖性舒张反应抑制的耐受性，并且这些舒张反应可适应血浆乙醇水平的存在，而血浆乙醇水平可能在体内抑制舒张。乙醇喂养大鼠舒张增强可能是对乙醇抑制作用产生耐受性的机制所致。
