Garofalo F A, Chang T M
Artificial Cells and Organs Research Center, Faculty of Medicine, McGill University, Montréal, Québec, Canada.
Appl Biochem Biotechnol. 1991 Jan;27(1):75-91. doi: 10.1007/BF02921517.
Pseudomonas pictorum in free or immobilized form can deplete serum cholesterol. The reaction kinetics control the global rate of cholesterol depletion during the initial part of free P. pictorum fermentation. Then, mass transfer takes control of the global rate. In this part of the fermentation, the global rate is first order with respect to the cholesterol concentration. The halftime was 330 min. The global rate is zero order with respect to the bacterial concentration. The activation energy was 83 kJ/mol. These results are consistent with the aqueous cholesterol diffusion model. The open pore agar microcapsule experimental effective diffusivity for lipoproteins at 37 degrees C was 6.7 x 10(-11) m2/s. Mass transfer across the microcapsule was not the limiting factor for the global rate.
游离或固定化形式的皮氏假单胞菌能够消耗血清胆固醇。反应动力学控制着游离皮氏假单胞菌发酵初期胆固醇消耗的整体速率。然后，传质控制整体速率。在发酵的这一阶段，整体速率相对于胆固醇浓度为一级反应。半衰期为330分钟。整体速率相对于细菌浓度为零级反应。活化能为83 kJ/mol。这些结果与水性胆固醇扩散模型一致。37℃下开孔琼脂微胶囊对脂蛋白的实验有效扩散率为6.7×10(-11) m2/s。微胶囊间的传质不是整体速率的限制因素。
