Quarfordt S H, Hilderman H L
J Lipid Res. 1970 Nov;11(6):528-35.
The exchange of free cholesterol in vitro between human red blood cells and low density lipoproteins (LDL) was quantified. The flux of sterol between LDL and red cells was relatively constant over a wide range of concentrations of free cholesterol in lipoproteins. In a system containing a suspension of red blood cells in a mixed solution of high density lipoproteins (HDL) and LDL, the fractional rate of exchange of HDL cholesterol was most rapid followed by LDL and lastly, by red cells. Increasing the ionic strength of the incubation media had no effect on the exchange of cholesterol between LDL and red cells. However, when both HDL and LDL were incubated with red cells in a buffer of increased ionic strength, total red cell cholesterol exchange was unaltered, but proportionately more exchange occurred with HDL and less with LDL. Addition of acetone to the buffer increased the exchange of cholesterol between LDL and red cells but produced no increment in red cell-HDL exchange.
对人红细胞与低密度脂蛋白（LDL）之间游离胆固醇的体外交换进行了定量分析。在脂蛋白中游离胆固醇浓度的广泛范围内，LDL与红细胞之间的甾醇通量相对恒定。在含有红细胞悬浮液的高密度脂蛋白（HDL）和LDL混合溶液系统中，HDL胆固醇的交换分数速率最快，其次是LDL，最后是红细胞。增加孵育介质的离子强度对LDL与红细胞之间的胆固醇交换没有影响。然而，当HDL和LDL在离子强度增加的缓冲液中与红细胞一起孵育时，总红细胞胆固醇交换未改变，但与HDL的交换比例增加，与LDL的交换比例减少。向缓冲液中添加丙酮增加了LDL与红细胞之间的胆固醇交换，但红细胞与HDL的交换没有增加。
