Köttgen E, Lindinger G, Reutter W
Clin Chim Acta. 1977 Oct 1;80(1):221-4. doi: 10.1016/0009-8981(77)90282-0.
The separation of two molecular forms of liver gamma-glutamyltransferase is achieved by Con A-Sepharose chromatography, an adult type with high affinity to Con A and a fetal type without binding capacity to this lectin. Now we present a new method using micro-columns for affinity chromatography (gel volume 2 ml). By this rapid and inexpensive procedure it is possible to study this enzyme separation for its use in the diagnosis of liver diseases.