用于γ-谷氨酰转移酶常规微量分析的伴刀豆球蛋白A-琼脂糖亲和层析法（作者译）
[Concanavalin A- Sepharose affinity chromatography for routine microanalysis of gamma-glutamyltransferase (author's transl)].
作者信息
Köttgen E, Lindinger G, Reutter W
出版信息
Clin Chim Acta. 1977 Oct 1;80(1):221-4. doi: 10.1016/0009-8981(77)90282-0.
PMID:20251
Abstract
The separation of two molecular forms of liver gamma-glutamyltransferase is achieved by Con A-Sepharose chromatography, an adult type with high affinity to Con A and a fetal type without binding capacity to this lectin. Now we present a new method using micro-columns for affinity chromatography (gel volume 2 ml). By this rapid and inexpensive procedure it is possible to study this enzyme separation for its use in the diagnosis of liver diseases.
摘要
通过刀豆球蛋白A-琼脂糖凝胶柱层析可分离出肝脏γ-谷氨酰转移酶的两种分子形式，一种是对刀豆球蛋白A具有高亲和力的成人型，另一种是对这种凝集素无结合能力的胎儿型。现在我们介绍一种使用微柱进行亲和层析的新方法（凝胶体积2毫升）。通过这种快速且经济的方法，可以研究这种酶的分离情况，以用于肝脏疾病的诊断。
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