• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

人胎盘NAD⁺依赖性15-羟基前列腺素脱氢酶保守酪氨酸151的定点诱变产生一种催化无活性的酶。

Site-directed mutagenesis of the conserved tyrosine 151 of human placental NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase yields a catalytically inactive enzyme.

作者信息

Ensor C M, Tai H H

机构信息

Division of Medicinal Chemistry and Pharmaceutics, College of Pharmacy, University of Kentucky, Lexington 40536-0082.

出版信息

Biochem Biophys Res Commun. 1991 Apr 30;176(2):840-5. doi: 10.1016/s0006-291x(05)80262-1.

DOI:10.1016/s0006-291x(05)80262-1
PMID:2025296
Abstract

NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase (15-PGDH) is a key enzyme involved in the biological inactivation of the prostaglandins. The cDNA for human placental 15-PGDH has been expressed in Escherichia coli. Site-directed mutagenesis was used to convert a highly conserved tyrosine at position 151 in 15-PGDH to an alanine. The DNA coding for this alanine mutant 15-PGDH was expressed in E. coli. Western blot analysis indicated that this mutant protein was expressed in amounts comparable to the wild type enzyme in bacteria, however no 15-PGDH activity could be detected. This result indicates that tyrosine 151 in 15-PGDH is essential for activity.

摘要

烟酰胺腺嘌呤二核苷酸（NAD⁺）依赖性15-羟基前列腺素脱氢酶（15-PGDH）是参与前列腺素生物失活的关键酶。人胎盘15-PGDH的cDNA已在大肠杆菌中表达。采用定点诱变将15-PGDH中第151位高度保守的酪氨酸转换为丙氨酸。编码该丙氨酸突变体15-PGDH的DNA在大肠杆菌中表达。蛋白质免疫印迹分析表明，该突变蛋白在细菌中的表达量与野生型酶相当，但未检测到15-PGDH活性。这一结果表明，15-PGDH中的酪氨酸151对活性至关重要。

相似文献

1
Site-directed mutagenesis of the conserved tyrosine 151 of human placental NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase yields a catalytically inactive enzyme.人胎盘NAD⁺依赖性15-羟基前列腺素脱氢酶保守酪氨酸151的定点诱变产生一种催化无活性的酶。
Biochem Biophys Res Commun. 1991 Apr 30;176(2):840-5. doi: 10.1016/s0006-291x(05)80262-1.
2
Bacterial expression and site-directed mutagenesis of two critical residues (tyrosine-151 and lysine-155) of human placental NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase.人胎盘NAD(+)依赖性15-羟基前列腺素脱氢酶两个关键残基（酪氨酸-151和赖氨酸-155）的细菌表达及定点诱变
Biochim Biophys Acta. 1994 Sep 21;1208(1):151-6. doi: 10.1016/0167-4838(94)90172-4.
3
Site-directed mutagenesis of the conserved serine 138 of human placental NAD+-dependent 15-hydroxyprostaglandin dehydrogenase to an alanine results in an inactive enzyme.将人胎盘烟酰胺腺嘌呤二核苷酸（NAD⁺）依赖性15-羟基前列腺素脱氢酶保守的丝氨酸138定点突变为丙氨酸会导致该酶失活。
Biochem Biophys Res Commun. 1996 Mar 18;220(2):330-3. doi: 10.1006/bbrc.1996.0405.
4
Threonine 188 is critical for interaction with NAD+ in human NAD+-dependent 15-hydroxyprostaglandin dehydrogenase.苏氨酸188对于人NAD+依赖性15-羟基前列腺素脱氢酶与NAD+的相互作用至关重要。
Biochem Biophys Res Commun. 1999 Apr 13;257(2):414-7. doi: 10.1006/bbrc.1999.0356.
5
Threonine 11 of human NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase may interact with NAD(+) during catalysis.人NAD(+)依赖性15-羟基前列腺素脱氢酶的苏氨酸11在催化过程中可能与NAD(+)相互作用。
Prostaglandins Leukot Essent Fatty Acids. 2002 May-Jun;66(5-6):505-9. doi: 10.1054/plef.2002.0391.
6
Expression of the cDNA for NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase as a catalytically active enzyme in Escherichia coli.
Prostaglandins Leukot Essent Fatty Acids. 1992 Jul;46(3):219-22. doi: 10.1016/0952-3278(92)90074-s.
7
Critical residues for the coenzyme specificity of NAD+-dependent 15-hydroxyprostaglandin dehydrogenase.NAD⁺依赖性15-羟基前列腺素脱氢酶辅酶特异性的关键残基。
Arch Biochem Biophys. 2003 Nov 15;419(2):139-46. doi: 10.1016/j.abb.2003.09.019.
8
Role of glutamine 148 of human 15-hydroxyprostaglandin dehydrogenase in catalytic oxidation of prostaglandin E2.人15-羟基前列腺素脱氢酶的谷氨酰胺148在前列腺素E2催化氧化中的作用
Bioorg Med Chem. 2006 Oct 1;14(19):6486-91. doi: 10.1016/j.bmc.2006.06.030. Epub 2006 Jul 7.
9
Cloning and expression of the cDNA for mouse NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase.
Biochim Biophys Acta. 1996 Nov 11;1309(1-2):21-4. doi: 10.1016/s0167-4781(96)00123-6.
10
Key NAD+-binding residues in human 15-hydroxyprostaglandin dehydrogenase.人15-羟基前列腺素脱氢酶中关键的烟酰胺腺嘌呤二核苷酸（NAD⁺）结合残基。
Arch Biochem Biophys. 2005 Jan 15;433(2):447-53. doi: 10.1016/j.abb.2004.09.036.

引用本文的文献

1
Structural Insights into Novel 15-Prostaglandin Dehydrogenase Inhibitors.新型 15-前列腺素脱氢酶抑制剂的结构见解。
Molecules. 2021 Sep 29;26(19):5903. doi: 10.3390/molecules26195903.
2
A Pterin-Dependent Signaling Pathway Regulates a Dual-Function Diguanylate Cyclase-Phosphodiesterase Controlling Surface Attachment in Agrobacterium tumefaciens.一条蝶呤依赖的信号通路调控一种双重功能的双鸟苷酸环化酶-磷酸二酯酶，该酶控制根癌土壤杆菌的表面附着。
mBio. 2015 Jun 30;6(4):e00156. doi: 10.1128/mBio.00156-15.
3
Chemistry of the retinoid (visual) cycle.
视黄醛（视觉）循环的化学过程。
Chem Rev. 2014 Jan 8;114(1):194-232. doi: 10.1021/cr400107q. Epub 2013 Jul 11.
4
Cryogenic and laser photoexcitation studies identify multiple roles for active site residues in the light-driven enzyme protochlorophyllide oxidoreductase.低温和激光光激发研究确定了光驱动酶原叶绿素酸氧化还原酶中活性位点残基的多种作用。
J Biol Chem. 2009 Jul 3;284(27):18160-6. doi: 10.1074/jbc.M109.020719. Epub 2009 May 13.
5
Aldo-keto reductases in which the conserved catalytic histidine is substituted.保守催化组氨酸被取代的醛酮还原酶。
Chem Biol Interact. 2009 Mar 16;178(1-3):127-33. doi: 10.1016/j.cbi.2008.10.046. Epub 2008 Nov 5.
6
Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12.编码大肠杆菌K-12中3-苯丙酸初始分解代谢双加氧途径的hca基因簇的特性分析
J Bacteriol. 1998 Jun;180(11):2915-23. doi: 10.1128/JB.180.11.2915-2923.1998.
7
Sequence analysis of the cloned glossy8 gene of maize suggests that it may code for a beta-ketoacyl reductase required for the biosynthesis of cuticular waxes.对克隆的玉米光泽8基因进行序列分析表明，它可能编码一种角质层蜡生物合成所需的β-酮酰还原酶。
Plant Physiol. 1997 Oct;115(2):501-10. doi: 10.1104/pp.115.2.501.
8
Cloning, nucleotide sequence, and overexpression of smoS, a component of a novel operon encoding an ABC transporter and polyol dehydrogenases of Rhodobacter sphaeroides Si4.球形红杆菌Si4中一个编码ABC转运蛋白和多元醇脱氢酶的新型操纵子的组成部分smoS的克隆、核苷酸序列及过表达
J Bacteriol. 1997 Oct;179(20):6335-40. doi: 10.1128/jb.179.20.6335-6340.1997.
9
Cloning, sequencing, and expression of isopropylbenzene degradation genes from Pseudomonas sp. strain JR1: identification of isopropylbenzene dioxygenase that mediates trichloroethene oxidation.来自假单胞菌属菌株JR1的异丙苯降解基因的克隆、测序及表达：介导三氯乙烯氧化的异丙苯双加氧酶的鉴定
Appl Environ Microbiol. 1996 Nov;62(11):3967-77. doi: 10.1128/aem.62.11.3967-3977.1996.
10
Characterization and expression of the plasmid-borne bedD gene from Pseudomonas putida ML2, which codes for a NAD+-dependent cis-benzene dihydrodiol dehydrogenase.恶臭假单胞菌ML2中编码NAD⁺依赖性顺式苯二氢二醇脱氢酶的质粒携带bedD基因的表征与表达。
J Bacteriol. 1996 Oct;178(19):5592-601. doi: 10.1128/jb.178.19.5592-5601.1996.