Bonnekoh B, Röding J, Krueger G R, Ghyczy M, Mahrle G
Department of Dermatology, University of Cologne, Germany.
Br J Dermatol. 1991 Apr;124(4):333-40. doi: 10.1111/j.1365-2133.1991.tb00593.x.
The in vitro effects of liposomes on HaCaT human keratinocytes were studied with regard to their uptake, lipid fluidity and proliferation of the cells. Oligolamellar liposomes, prepared from soya bean phospholipids, had a mean size of 150 mm and consisted predominantly of phosphatidylcholine (83%) and phosphatidylethanolamine (10%) and the fatty acids comprised mainly linoleic acid (66%) or other unsaturated fatty acids. After 6 and 24 h of incubation with 1 and 0.1% w/v of liposomal lipids, phase-contrast microscopy revealed marked cytoplasmic vacuolization of the cells. Keratinocytes treated with the liposomes contained aggregations of multilaminated lipid material without delimiting cell membranes. The cellular lipid fluidity (reciprocal of diphenylhexatriene fluorescence polarization P-value) correlated with liposomal concentration and incubation time. A significant elevation of lipid fluidity (P less than 0.05) was observed with 1 and 0.1% liposomes after 1 h of incubation (81.8 +/- 4.7 and 95.7 +/- 1.2% of control P value) and for 0.01% liposomes after 3 h (96.2 +/- 1.5%). Maximum fluidity occurred after 48 h of exposure to 1% liposomes (42.1 +/- 3.1%). Exposure to liposomal lipids for 24 and 48 h resulted in suppressed cell proliferation with 50% inhibition concentrations (IC50), being 0.06% for incorporation of [3H]-thymidine. 0.08% for [14C]-amino-acid incorporation and greater than 1% for protein content per well after 24 h of exposure. The cells were able to proliferate and lipid fluidity returned to normal within 7 days following discontinuation of incubation with liposomal lipids.
研究了脂质体对HaCaT人角质形成细胞的体外作用，涉及细胞摄取、脂质流动性和细胞增殖。由大豆磷脂制备的寡层脂质体平均大小为150nm，主要由磷脂酰胆碱（83%）和磷脂酰乙醇胺（10%）组成，脂肪酸主要包括亚油酸（66%）或其他不饱和脂肪酸。在用1%和0.1%w/v的脂质体脂质孵育6小时和24小时后，相差显微镜显示细胞出现明显的细胞质空泡化。用脂质体处理的角质形成细胞含有多层脂质物质聚集体，没有界定的细胞膜。细胞脂质流动性（二苯基己三烯荧光偏振P值的倒数）与脂质体浓度和孵育时间相关。孵育1小时后，1%和0.1%脂质体的脂质流动性显著升高（P小于0.05）（分别为对照P值的81.8±4.7%和95.7±1.2%），0.01%脂质体孵育3小时后脂质流动性显著升高（96.2±1.5%）。暴露于1%脂质体48小时后出现最大流动性（42.1±3.1%）。暴露于脂质体脂质24小时和48小时导致细胞增殖受到抑制，50%抑制浓度（IC50），[3H] - 胸腺嘧啶掺入为0.06%，[14C] - 氨基酸掺入为0.08%，暴露24小时后每孔蛋白质含量大于1%。在停止与脂质体脂质孵育后7天内，细胞能够增殖，脂质流动性恢复正常。
