Gavel Y, Steppuhn J, Herrmann R, von Heijne G
Department of Theoretical Physics, Royal Institute of Technology, Stockholm, Sweden.
FEBS Lett. 1991 Apr 22;282(1):41-6. doi: 10.1016/0014-5793(91)80440-e.
In integral membrane proteins, regions that span the lipid bilayer alternate with regions that are exposed on either side of the membrane. For proteins from the plasma membrane of both prokaryotic and eukaryotic cells it has been shown that the exposed parts follow a 'positive-inside rule': on average, segments that are translocated across the membrane have a 2-4-fold lower frequency of positively charged residues than non-translocated segments. We now present an analysis of proteins from the thylakoid membrane of chloroplasts. It is shown that these proteins have the same charge asymmetry as has been reported for proteins from other membrane systems, with their more highly charged regions facing the stromal compartment.
在整合膜蛋白中，跨越脂质双层的区域与暴露于膜两侧的区域交替出现。对于原核细胞和真核细胞质膜上的蛋白质，研究表明，暴露部分遵循“内侧为正规则”：平均而言，跨膜转运的片段中带正电荷残基的频率比未转运片段低2至4倍。我们现在对叶绿体类囊体膜上的蛋白质进行分析。结果表明，这些蛋白质具有与其他膜系统中蛋白质相同的电荷不对称性，其电荷较高的区域面向基质区室。
