Hirabayashi Y, Tamura S I, Suzuki Y, Nagamine T, Aizawa C, Shimada K, Kurata T
Department of Pathology, National Institute of Health, Tokyo, Japan.
Immunology. 1991 Mar;72(3):329-35.
Cholera toxin B subunit (CTB) has been shown to augment the antibody responses to influenza virus haemagglutinin (HA) in BALB/c mice immunized with HA vaccine together with CTB. In this study, mouse strain differences in the adjuvant effect of CTB on anti-HA antibody responses were investigated along with those in the antibody responses to CTB or HA, using various inbred and H-2 congenic strains. The antibody responsiveness to CTB depended on the H-2 haplotype of the strain: strains with the H-2b haplotype were high responders, those with H-2a, H-2k and H-2s were low responders, and those with H-2d were intermediate. The responsiveness to HA was also related to the H-2 haplotype: H-2a and H-2k strains were high responders, H-2b and H-2s strains were low responders, and H-2d strains were intermediate. However, the degree of the adjuvant effect of CTB on anti-HA antibody responses was almost constant, regardless of the H-2 haplotype or other genetic backgrounds of the strain. The lack of genetic restriction of the adjuvant effect would be favourable for application of CTB-combined HA vaccine to humans, who are genetically diverse. Moreover, these results suggest that the immunogenicity and adjuvanticity of CTB differ essentially in their mechanisms.
霍乱毒素B亚基（CTB）已被证明可增强用HA疫苗和CTB免疫的BALB/c小鼠对流感病毒血凝素（HA）的抗体反应。在本研究中，使用各种近交系和H-2同源系，研究了CTB对HA抗体反应的佐剂效应在小鼠品系中的差异以及对CTB或HA的抗体反应差异。对CTB的抗体反应性取决于品系的H-2单倍型：具有H-2b单倍型的品系是高反应者，具有H-2a、H-2k和H-2s的品系是低反应者，具有H-2d的品系是中等反应者。对HA的反应性也与H-2单倍型有关：H-2a和H-2k品系是高反应者，H-2b和H-2s品系是低反应者，H-2d品系是中等反应者。然而，无论品系的H-2单倍型或其他遗传背景如何，CTB对HA抗体反应的佐剂效应程度几乎是恒定的。佐剂效应缺乏遗传限制将有利于CTB联合HA疫苗应用于遗传背景多样的人类。此外，这些结果表明CTB的免疫原性和佐剂性在机制上基本不同。
