Ryffel B, Woerly G, Greiner B, Haendler B, Mihatsch M J, Foxwell B M
Sandoz Pharma Ltd, Drug Safety Assessment, Basle, Switzerland.
Immunology. 1991 Mar;72(3):399-404.
Cyclophilin (CYP) is the major intracellular binding protein for the immunosuppressive drug cyclosporine (CS). CYP distribution was investigated in human tissues by solid-phase immunoassay, Western and Northern blot analysis as well as immunohistochemistry. CYP was found in all tissues examined at concentrations in the range of 1 microgram/mg protein. Furthermore, mRNA specific for CYP was found in every tissue, indicating local production of the protein. Immunohistochemical investigations revealed preferential parenchymal and only little stromal localization. Within certain organs, e.g. kidneys, regional differences of immunoreactive CYP was evident. The presence of CYP was also investigated in several lymphoid and non-lymphoid cell lines and was found at comparable concentrations. Immunogold staining confirmed cytosolic, but revealed also nuclear localization of CYP. The possible role of this abundant CS binding protein is discussed.
亲环蛋白（CYP）是免疫抑制药物环孢素（CS）的主要细胞内结合蛋白。通过固相免疫测定、蛋白质印迹和Northern印迹分析以及免疫组织化学研究了CYP在人体组织中的分布。在所检测的所有组织中均发现了CYP，其浓度范围为1微克/毫克蛋白质。此外，在每个组织中都发现了CYP特异性的mRNA，表明该蛋白可在局部产生。免疫组织化学研究显示，CYP主要定位于实质细胞，而在基质细胞中定位较少。在某些器官（如肾脏）内，免疫反应性CYP存在明显的区域差异。还研究了几种淋巴细胞系和非淋巴细胞系中CYP的存在情况，发现其浓度相当。免疫金染色证实CYP存在于细胞质中，但也显示其存在于细胞核中。文中讨论了这种丰富的CS结合蛋白可能发挥的作用。
