Haendler B, Hofer-Warbinek R, Hofer E
EMBO J. 1987 Apr;6(4):947-50. doi: 10.1002/j.1460-2075.1987.tb04843.x.
Complementary DNA encoding human cyclophilin, a specific cyclosporin A-binding protein, has been isolated from the leukemic T-cell line Jurkat and sequenced. Comparison of the deduced amino acid sequence with the previously determined sequence of bovine thymus cyclophilin reveals only three differences: an additional amino acid at the carboxy terminus end and two internal changes. RNA transfer blot analysis indicates an mRNA size of approximately 1 kb for human T-cell cyclophilin. Phytohaemagglutinin and phorbol myristate acetate induction of T cells treated or not with cyclosporin A affects only marginally the level of cyclophilin mRNA. Southern blot analysis of human genomic DNA digested with different restriction enzymes strongly suggests the existence of a multigene family for cyclophilin.
编码人亲环蛋白（一种特异性结合环孢菌素A的蛋白质）的互补DNA已从白血病T细胞系Jurkat中分离出来并进行了测序。将推导的氨基酸序列与先前确定的牛胸腺亲环蛋白序列进行比较，仅发现三个差异：羧基末端有一个额外的氨基酸以及两个内部变化。RNA转移印迹分析表明，人T细胞亲环蛋白的mRNA大小约为1 kb。用环孢菌素A处理或未处理的T细胞经植物血凝素和佛波酯诱导，仅对亲环蛋白mRNA水平有轻微影响。用不同限制酶消化的人基因组DNA的Southern印迹分析强烈表明存在亲环蛋白多基因家族。
