Angulo J A, Ledoux M, McEwen B S
Laboratory of Neuroendocrinology, Rockefeller University, New York, New York 10021.
J Neurochem. 1991 Jun;56(6):2033-8. doi: 10.1111/j.1471-4159.1991.tb03463.x.
We assessed the effects of cold and isolation stress on arginine vasopressin (AVP) mRNA in the paraventricular (PVN) and supraoptic (SON) nuclei of the hypothalamus. Vasopressin mRNA levels were determined by in situ hybridization histochemistry at the cellular level. In posterior magnocellular neurons of the PVN isolation stress for 7 or 14 days increased vasopressin mRNA levels 28 and 29%, respectively, compared to group-housed controls. No significant alterations in vasopressin gene expression were observed in the SON after 7 or 14 days of isolation stress. Scattered magnocellular AVP mRNA-expressing cells of the medial parvocellular PVN showed increases of 19 and 34% after 7 and 14 days of isolation, respectively. We also studied the effect of cold or combined cold and isolation stress on vasopressin gene expression in the PVN and SON. Cold stress for 3 h daily for 4 consecutive days increased AVP mRNA levels in the posterior magnocellular PVN by 15%. Cold-isolated animals showed an increase of 21%. No significant effect on AVP mRNA levels in the SON was observed. In contrast to the posterior magnocellular PVN, cold or cold-isolation stress increased AVP mRNA in magnocellular neurons of the medial parvocellular region of the PVN by 25 and 43%, respectively, relative to control rats. These results suggest that psychological and metabolic stress may be added to the list of stressors that activate the hypothalamo-neurohypophysial system.
我们评估了寒冷和隔离应激对下丘脑室旁核（PVN）和视上核（SON）中精氨酸加压素（AVP）mRNA的影响。通过细胞水平的原位杂交组织化学来测定加压素mRNA水平。与群居对照相比，在PVN的后大细胞神经元中，7天或14天的隔离应激分别使加压素mRNA水平提高了28%和29%。在隔离应激7天或14天后，SON中未观察到加压素基因表达的显著变化。内侧小细胞PVN中散在的表达大细胞AVP mRNA的细胞在隔离7天和14天后分别增加了19%和34%。我们还研究了寒冷或寒冷与隔离联合应激对PVN和SON中加压素基因表达的影响。连续4天每天3小时的寒冷应激使后大细胞PVN中的AVP mRNA水平提高了15%。寒冷隔离的动物增加了21%。未观察到对SON中AVP mRNA水平有显著影响。与后大细胞PVN不同，相对于对照大鼠，寒冷或寒冷隔离应激分别使PVN内侧小细胞区域的大细胞神经元中的AVP mRNA增加了25%和43%。这些结果表明，心理和代谢应激可能被添加到激活下丘脑 - 神经垂体系统的应激源列表中。
