从BALB/c小鼠的自发性非产毒株骨肉瘤中拯救转化病毒。

Rescue of a transforming virus from a spontaneous nonproducing osteosarcoma in BALB/c mice.

作者信息

Bentvelzen P, Nooter K, Deys B F

出版信息

J Natl Cancer Inst. 1978 Feb;60(2):401-3. doi: 10.1093/jnci/60.2.401.

DOI:10.1093/jnci/60.2.401

PMID:202717

Abstract

Cells from spontaneous osteosarcoma V793 that originated in a 19-month-old female BALB/c mouse were cultured. They did not produce a C-type oncovirus as determined by extracellular reverse transcriptase assay and cytoplasmic immunofluorescence. After cocultivation with Balb/3T3 cells chronically infected with a murine leukemia virus (MuLV), a focus-forming principle that transformed 3T3 cells, secondary BALB/c mouse embryo and WAG/Rij rat embryo fibroblasts were rescued. The transformation could be inhibited by antiserum to MuLV.

摘要

对源自一只19个月大雌性BALB/c小鼠的自发性骨肉瘤V793的细胞进行培养。通过细胞外逆转录酶测定和细胞质免疫荧光测定，发现它们不产生C型肿瘤病毒。在与长期感染鼠白血病病毒（MuLV）的Balb/3T3细胞共培养后，一种能转化3T3细胞的致瘤原理被挽救出来，该原理还能使二代BALB/c小鼠胚胎和成纤维细胞以及WAG/Rij大鼠胚胎成纤维细胞发生转化。这种转化可被抗MuLV血清抑制。