Merz H, Rytik G, Müller W E, Röder W
Abteilung Angewandte Molekularbiologie, Institut für Physiologische Chemie der Johannes Gutenberg-Universität Mainz.
Unfallchirurg. 1991 Jan;94(1):47-9.
The human immunodeficiency virus type 1 (HIV-1) is harboured not only in lymphocytes and macrophages but also in other cells, e.g. brain cells. Hence, it is not surprising that HIV-1 is also present in bone of HIV-infected individuals. Here we present an assay system for detection of the virus in human bone pieces in vitro. Bone pieces (i) from uninfected individuals, which had been incubated with cell-free virus preparations or cells producing the virus, or (ii) from and AIDS patient were used. These specimens were coincubated with CEM cells, which in turn became infected; the infection was traced immunocytochemically and enzymatically. In addition, we show that infection of CEM cells by HIV-1 from bone of an AIDS patient in vitro can be blocked by Sulfoevernan, a compound recently discovered by our group.
1型人类免疫缺陷病毒（HIV-1）不仅存在于淋巴细胞和巨噬细胞中，还存在于其他细胞中，例如脑细胞。因此，HIV-1也存在于HIV感染个体的骨骼中也就不足为奇了。在此，我们展示了一种用于体外检测人类骨块中该病毒的检测系统。使用了以下骨块：（i）来自未感染个体的骨块，这些骨块已与无细胞病毒制剂或产生病毒的细胞一起孵育，或（ii）来自一名艾滋病患者的骨块。将这些标本与CEM细胞共同孵育，CEM细胞继而被感染；通过免疫细胞化学和酶学方法追踪感染情况。此外，我们还表明，体外来自艾滋病患者骨骼的HIV-1对CEM细胞的感染可被磺基埃弗南阻断，磺基埃弗南是我们团队最近发现的一种化合物。
