对小鼠肝癌和可的松刺激的大鼠肝脏中聚腺苷酸加尾RNA（poly(A+) -RNA）的研究。

Studies of poly(A+)-RNA in mouse hepatoma and cortisone-stimulated rat liver.

作者信息

Konstantinova I M, Kulichkova V A, Vorob'ev V I, Ratovitski E A, Shaposhnikov J D

出版信息

Mol Cell Biochem. 1977 Nov 25;18(1):29-32. doi: 10.1007/BF00215276.

PMID:202862

Abstract

The content of poly(A)-containing RNA in subcellar fractions has been investigated both in cortisone-treated rat liver and experimental hepatoma cells. The fractions included nuclei, cytoplasm, mitochondria, free and membrane-bound polyribosomes. 1) In both cases of genome activation (cortisone induction and hepatoma cells) an increase in poly(A) content of all subcellular fractions except free polyribosomes was observed. 2) Cortisone was found to induce elongation of poly(A) segments detected in both nuclei and cytoplasm. 3) An increase in the poly(A) block size also was found to be stimulated in nuclei and cytoplasm of hepatoma cells. 4) The observed elongation in poly(A) length occurred against the background of an increase of the population of of poly(A)-RNA's.

摘要

已对经可的松处理的大鼠肝脏和实验性肝癌细胞亚细胞组分中含聚腺苷酸（poly(A)）的RNA含量进行了研究。这些组分包括细胞核、细胞质、线粒体、游离和膜结合的多核糖体。1）在基因组激活的两种情况下（可的松诱导和肝癌细胞），除游离多核糖体外，所有亚细胞组分的聚腺苷酸含量均增加。2）发现可的松可诱导在细胞核和细胞质中检测到的聚腺苷酸片段延长。3）还发现肝癌细胞核和细胞质中的聚腺苷酸块大小增加受到刺激。4）观察到的聚腺苷酸长度延长是在聚腺苷酸RNA群体增加的背景下发生的。