一种用于制备大鼠肝脏膜结合核糖体的改进细胞分级分离程序。
An improved cell fractionation procedure for the preparation of rat liver membrane-bound ribosomes.
作者信息
Adelman M R, Blobel G, Sabatini D D
出版信息
J Cell Biol. 1973 Jan;56(1):191-205. doi: 10.1083/jcb.56.1.191.
Abstract
A cell fractionation procedure is described which allows the preparation from rat liver of a rough microsome population containing almost 50% of the membrane-bound ribosomes of the tissue. The fraction is not contaminated with free ribosomes or smooth microsomes, and, by various other criteria, is suitable for studies of ribosome-membrane interaction.
摘要
本文描述了一种细胞分级分离程序，该程序可从大鼠肝脏中制备出一种粗微粒体群体，其包含该组织中近50%的膜结合核糖体。该级分未被游离核糖体或滑面微粒体污染，并且根据各种其他标准，适用于核糖体-膜相互作用的研究。
相似文献
1
An improved cell fractionation procedure for the preparation of rat liver membrane-bound ribosomes.一种用于制备大鼠肝脏膜结合核糖体的改进细胞分级分离程序。
J Cell Biol. 1973 Jan;56(1):191-205. doi: 10.1083/jcb.56.1.191.
2
Ribosome-membrane interaction. Nondestructive disassembly of rat liver rough microsomes into ribosomal and membranous components.核糖体与膜的相互作用。大鼠肝脏粗面微粒体无损拆解为核糖体和膜成分。
J Cell Biol. 1973 Jan;56(1):206-29. doi: 10.1083/jcb.56.1.206.
3
Electron microscope examination of subcellular fractions. 3. Quantitative analysis of the microsomal fraction isolated from rat liver.亚细胞组分的电子显微镜检查。3. 从大鼠肝脏分离的微粒体组分的定量分析。
J Cell Biol. 1971 Oct;51(1):52-71. doi: 10.1083/jcb.51.1.52.
4
Zonal centrifugation of microsomes from rat liver: resolution of rough- and smooth-surfaced membranes.大鼠肝脏微粒体的区带离心：糙面和光面内质网膜的分离
Biochem Biophys Res Commun. 1969 Aug 22;36(5):748-55. doi: 10.1016/0006-291x(69)90673-1.
5
Subfractionation of smooth microsomes from rat liver.大鼠肝脏光滑微粒体的亚分级分离
J Cell Biol. 1970 Oct;47(1):34-48. doi: 10.1083/jcb.47.1.34.
6
Effect of ribosome removal of rough microsomal composition and polysome attachment in vitro. Comparison of liver and kidney.体外核糖体去除对粗面微粒体组成和多核糖体附着的影响。肝脏与肾脏的比较。
Biochim Biophys Acta. 1974 Nov 20;374(1):59-75. doi: 10.1016/0005-2787(74)90199-3.
7
Nondestructive separation of rat liver rough microsomes into ribosomal and membranous components.大鼠肝脏粗面微粒体无损分离为核糖体和膜成分。
Methods Enzymol. 1974;31:201-15. doi: 10.1016/0076-6879(74)31022-1.
8
9
A comparative study of the molecular structures of the plasma membranes and the smooth and the rough endoplasmic-reticulum membranes from rat liver.大鼠肝脏质膜、滑面内质网膜和粗面内质网膜分子结构的比较研究。
Biochem J. 1972 Sep;129(3):781-8. doi: 10.1042/bj1290781.
10
Isolation and properties of polyribosomes and fragments of the endoplasmic reticulum from rat liver.大鼠肝脏多核糖体和内质网片段的分离及特性
Biochem J. 1967 Apr;103(1):177-82. doi: 10.1042/bj1030177.
引用本文的文献
1
Insulin activates intracellular transport of lipid droplets to release triglycerides from the liver.胰岛素激活细胞内脂滴运输，将甘油三酯从肝脏中释放出来。
J Cell Biol. 2019 Nov 4;218(11):3697-3713. doi: 10.1083/jcb.201903102. Epub 2019 Oct 11.
2
Visualization of cytosolic ribosomes on the surface of mitochondria by electron cryo-tomography.电子冷冻断层扫描技术可视化细胞质核糖体在线粒体表面的分布。
EMBO Rep. 2017 Oct;18(10):1786-1800. doi: 10.15252/embr.201744261. Epub 2017 Aug 21.
3
Phytochrome and phosphotungstate-chromate-positive vesicles from Cucurbita pepo L.南瓜叶的光敏色素和磷钨酸铬酸盐阳性小泡
Planta. 1977 Jan;133(2):169-77. doi: 10.1007/BF00391916.
4
In vitro dissection of protein translocation into the mammalian endoplasmic reticulum.蛋白质转运至哺乳动物内质网的体外剖析
Methods Mol Biol. 2010;619:339-63. doi: 10.1007/978-1-60327-412-8_20.
5
Polyribosomes from Peas: V. An Attempt to Characterize the Total Free and Membrane-bound Polysomal Population.豌豆多核糖体：V. 尝试对总游离和膜结合多核糖体群体进行特征分析。
Plant Physiol. 1975 Apr;55(4):749-56. doi: 10.1104/pp.55.4.749.
6
Active translocon complexes labeled with GFP-Dad1 diffuse slowly as large polysome arrays in the endoplasmic reticulum.用绿色荧光蛋白标记的Dad1（GFP-Dad1）标记的活性易位子复合物在内质网中作为大的多核糖体阵列缓慢扩散。
J Cell Biol. 2002 Aug 5;158(3):497-506. doi: 10.1083/jcb.200201116.
7
The 170-kDa glucose-regulated stress protein is an endoplasmic reticulum protein that binds immunoglobulin.170千道尔顿的葡萄糖调节应激蛋白是一种结合免疫球蛋白的内质网蛋白。
Mol Biol Cell. 1993 Nov;4(11):1109-19. doi: 10.1091/mbc.4.11.1109.
8
Cytochrome P450IID6 recognized by LKM1 antibody is not exposed on the surface of hepatocytes.被LKM1抗体识别的细胞色素P450IID6并不暴露在肝细胞表面。
Clin Exp Immunol. 1993 Jun;92(3):381-90. doi: 10.1111/j.1365-2249.1993.tb03409.x.
9
Intracellular distribution of Sindbis virus membrane proteins in BHK-21 cells infected with wild-type virus and maturation-defective mutants.辛德毕斯病毒膜蛋白在感染野生型病毒和成熟缺陷型突变体的BHK-21细胞中的细胞内分布。
J Virol. 1980 Dec;36(3):775-86. doi: 10.1128/JVI.36.3.775-786.1980.
10
Characterization of a small, nonstructural viral polypeptide present late during infection of BHK cells by Semliki Forest virus.对塞姆利基森林病毒感染BHK细胞后期出现的一种小的非结构病毒多肽的特性分析。
J Virol. 1980 Jan;33(1):230-7. doi: 10.1128/JVI.33.1.230-237.1980.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Nuclei from normal and leukemic mouse spleen. I. The isolation of nuclei in neutral medium.正常和白血病小鼠脾脏的细胞核。I. 在中性介质中细胞核的分离。
Cancer Res. 1950 Dec;10(12):751-4.
3
THE ESTIMATION OF DEOXYRIBONUCLEIC ACID IN THE PRESENCE OF SIALIC ACID: APPLICATION TO ANALYSIS OF HUMAN GASTRIC WASHINGS.在唾液酸存在的情况下对脱氧核糖核酸的估计：在人胃冲洗液分析中的应用。
Biochem J. 1965 Jun;95(3):612-20. doi: 10.1042/bj0950612.
4
CHARACTERIZATION OF RIBOSOMAL AGGREGATES ISOLATED FROM LIVER.从肝脏中分离出的核糖体聚集体的特性分析
Proc Natl Acad Sci U S A. 1964 Nov;52(5):1241-8. doi: 10.1073/pnas.52.5.1241.
5
The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimation.施密特 - 坦豪泽核酸估测法中紫外线吸收测量的精度。
Biochim Biophys Acta. 1962 May 14;55:571-83. doi: 10.1016/0006-3002(62)90836-3.
6
Characterization of uterine peroxidase.子宫过氧化物酶的特性分析
J Biol Chem. 1958 Jul;233(1):206-8.
7
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.用于比色法测定脱氧核糖核酸的二苯胺反应的条件及机制研究。
Biochem J. 1956 Feb;62(2):315-23. doi: 10.1042/bj0620315.
8
Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue.组织分级分离研究。6. 大鼠肝脏组织中酶的细胞内分布模式。
Biochem J. 1955 Aug;60(4):604-17. doi: 10.1042/bj0600604.
9
Electron microscopic examination of subcellular fractions. I. The preparation of representative samples from suspensions of particles.亚细胞组分的电子显微镜检查。I. 从颗粒悬浮液中制备代表性样品。
J Cell Biol. 1967 Jan;32(1):181-91. doi: 10.1083/jcb.32.1.181.
10
Studies on free and membrane-bound ribosomes in rat liver. II. Interaction of ribosomes and membranes.大鼠肝脏中游离核糖体与膜结合核糖体的研究。II. 核糖体与膜的相互作用。
J Mol Biol. 1967 Jun 14;26(2):293-301. doi: 10.1016/0022-2836(67)90298-7.
Zotero 插件