Minami N, Tani E, Yokota M, Maeda Y, Yamaura I
Department of Neurosurgery, Hyogo College of Medicine, Japan.
Acta Neuropathol. 1991;81(4):401-7. doi: 10.1007/BF00293461.
Experimental cerebral vasospasm was produced in a "two-hemorrhage" canine model and examined by immunohistochemistry for leukotriene C4 (LTC4). The immunostain for LTC4 showed a strong positivity in intima and adventitia and a scattered reaction in media of normal basilar artery. The immunoreactivity after subarachnoid hemorrhage (SAH) was little changed in intima and media. Inflammatory cells which were characterized histochemically as neutrophils and macrophages, were shown to infiltrate from the adventitia of basilar artery to the periphery of blood clot after SAH and were markedly immunoreactive for LTC4. Also the neutrophils increased in number with the lapse of time after SAH. Thus, it would be reasonable to conclude that the LTC4 responsible for the development of vasospasm would most likely be produced from the infiltrating neutrophils and macrophages. In addition, neurons in hypothalamus, median eminence, and pons, as well as ependymal and arachnoid cells were immunoreactive for LTC4 both in the control and after SAH, whereas astrocytes and oligodendrocytes were not immunoreactive for LTC4 in either case.
在“两次出血”犬模型中诱导实验性脑血管痉挛，并通过免疫组织化学检测白三烯C4（LTC4）。LTC4的免疫染色显示在正常基底动脉的内膜和外膜呈强阳性，在中膜呈散在反应。蛛网膜下腔出血（SAH）后，内膜和中膜的免疫反应性变化不大。组织化学特征为中性粒细胞和巨噬细胞的炎性细胞，在SAH后从基底动脉外膜浸润到血凝块周边，并且对LTC4呈明显免疫反应性。此外，中性粒细胞数量在SAH后随时间增加。因此，可以合理地得出结论，导致血管痉挛发生的LTC4很可能由浸润的中性粒细胞和巨噬细胞产生。此外，在对照和SAH后，下丘脑、正中隆起和脑桥中的神经元以及室管膜细胞和蛛网膜细胞对LTC4均呈免疫反应性，而星形胶质细胞和少突胶质细胞在两种情况下对LTC4均无免疫反应性。
