Yokota M, Tani E, Maeda Y
Department of Neurosurgery, Hyogo College of Medicine, Japan.
Stroke. 1989 Apr;20(4):527-33. doi: 10.1161/01.str.20.4.527.
We produced cerebral vasospasm in 29 dogs by the "two-hemorrhage" method of intracisternal injections, 2 days apart, of autogenous arterial blood. Leukotriene (LT) C4, LTD4, and LTE4 were purified from incubated basilar artery, medulla oblongata, hypothalamus, median eminence, and blood clot from around the basilar artery using reverse-phase high-performance liquid chromatography, and the amount of each LT was quantified separately by bioassay with guinea pig ileum. The biosynthetic capacity for total LTs was approximately three times higher in the hypothalamus and median eminence than in the basilar artery and medulla oblongata in the eight normal dogs. In the dogs with subarachnoid hemorrhage, the biosynthetic capacity was increased significantly both before and 2 hours after the second injection of blood on Day 2 and was normal on Day 7 in the basilar artery and medulla oblongata, whereas the biosynthetic capacity was decreased significantly 2 hours after the first and second injections of blood and was increased significantly on Day 7 in the hypothalamus and median eminence. In blood clot the biosynthetic capacity was increased continuously after the first injection of blood. Thus, the biosynthetic capacity for total LTs showed a time- and tissue-specific change after subarachnoid hemorrhage.
我们采用“两次出血”法，于蛛网膜下腔内间隔2天注射自体动脉血，在29只犬中诱导出脑血管痉挛。使用反相高效液相色谱法从孵育的基底动脉、延髓、下丘脑、正中隆起以及基底动脉周围的血凝块中纯化白三烯（LT）C4、LTD4和LTE4，并通过豚鼠回肠生物测定法分别对每种LT的量进行定量。在8只正常犬中，下丘脑和正中隆起中总LTs的生物合成能力比基底动脉和延髓中的大约高三倍。在蛛网膜下腔出血的犬中，第2天第二次注射血液前和注射后2小时，基底动脉和延髓中的生物合成能力显著增加，第7天恢复正常；而在下丘脑和正中隆起中，第一次和第二次注射血液后2小时生物合成能力显著降低，第7天显著增加。在血凝块中，第一次注射血液后生物合成能力持续增加。因此，蛛网膜下腔出血后总LTs的生物合成能力呈现出时间和组织特异性变化。
