Fix A S, Arp L H
Department of Veterinary Pathology, Iowa State University, Ames 50011.
Avian Dis. 1991 Jan-Mar;35(1):174-9.
Tracer particle uptake by conjunctiva-associated lymphoid tissue (CALT) was quantified in the lower eyelids of 1-, 3-, 5-, and 7-week-old broiler chickens. CALT was measured histologically by computerized image analysis in all birds and in additional 1-day-old and 16-week-old chickens not subjected to uptake assessment. Suspensions of carbon or iron oxide were placed in contact with CALT for 5, 15, or 30 minutes (contact time). After eyelid removal, tracer uptake was scored by light microscopy, CALT was measured, and a mathematically derived uptake index was evaluated statistically. At each age examined, computer-generated measurements showed a significant increase in the proportion of CALT lymphoepithelium within proximal eyelids. Within the conjunctival sites evaluated, tracer uptake was significantly greater in lymphoepithelium than in non-lymphoepithelium at all ages and at all contact times. Uptake increased significantly between 3 and 5 weeks of age and between 5 and 15 minutes of tracer contact. Based on these uptake data for CALT in chickens, a minimal age-specific maturity is suggested that may influence function in mucosal immunity.
在1周龄、3周龄、5周龄和7周龄的肉鸡下眼睑中，对结膜相关淋巴组织（CALT）摄取示踪颗粒的情况进行了定量分析。通过计算机图像分析对所有鸡以及另外未进行摄取评估的1日龄和16周龄鸡的CALT进行了组织学测量。将碳或氧化铁悬浮液与CALT接触5分钟、15分钟或30分钟（接触时间）。去除眼睑后，通过光学显微镜对示踪剂摄取情况进行评分，测量CALT，并对通过数学推导得出的摄取指数进行统计学评估。在每个检查的年龄组中，计算机生成的测量结果显示近端眼睑内CALT淋巴上皮的比例显著增加。在所评估的结膜部位，在所有年龄和所有接触时间下，淋巴上皮中的示踪剂摄取均显著高于非淋巴上皮。在3至5周龄之间以及示踪剂接触5至15分钟之间，摄取量显著增加。基于鸡CALT的这些摄取数据，提示了一个可能影响黏膜免疫功能的最小年龄特异性成熟度。
