利用腺苷酸激酶和[γ-32P]ATP进行[β-32P]ADP的酶促合成。
Enzymatic synthesis of [beta-32P]ADP using adenylate kinase and [gamma-32P]ATP.
作者信息
Dawson J M, Cook N D, Peters T J
出版信息
Anal Biochem. 1985 Sep;149(2):471-3. doi: 10.1016/0003-2697(85)90600-1.
PMID:3000219
Abstract
An enzymatic method for the synthesis of [beta-32P]ADP from [gamma-32P]ATP is described. This substrate is required for the assay of ADPase and is not commercially available. The method described results in a preparation of [beta-32P]ADP of high purity with a yield of approximately 40% the theoretical obtainable.
摘要
本文描述了一种从[γ-32P]ATP合成[β-32P]ADP的酶促方法。这种底物是测定ADP酶所必需的，且没有商业供应。所描述的方法可制备出高纯度的[β-32P]ADP，产率约为理论可得量的40%。
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