Wang L H, Jiang Z S, Chen Z F
Second Military Medical College, Shanghai.
Yi Chuan Xue Bao. 1991;18(1):90-6.
Sixty kinds of commonly used Chinese medicines have been examined for their ability to depress the release of Lambda phage from lysogenic strain in the inductest. 11 Chinese medicines showed an inhibitory effects. Among them, Codonopsis radix, Polygonatum radix and fractus Lycium were strong depressors. They also showed an inhibitory effect on SOS response in SOS chromotest with a dose-effect response. These medicines were also found to decrease the frequency of gene conversion in S. cerevisiae in the presence of hydroxyurea. The effective compound (s) of Polygonatum radix partially purified from the extract with Sephadex G-25 chromatography was a reductive carbohydrate with molecular weight less than 3,000. The compound was shown to exert an inhibitory effect on SOS response occurred at 42 degrees C in E. coli GW1060 (recA441), but has no effect on SOS network gene expression in E. coli GW 1107 (lexA51), suggesting that Polygonatum radix may contain an inhibitor of RecA protease.
已对60种常用中药进行检测，以考察其在诱导试验中抑制溶源菌释放λ噬菌体的能力。11种中药表现出抑制作用。其中，党参、玉竹和枸杞子是强效抑制剂。它们在SOS显色试验中对SOS反应也表现出剂量效应的抑制作用。还发现这些药物在有羟基脲存在的情况下可降低酿酒酵母中的基因转换频率。用葡聚糖凝胶G - 25柱色谱从玉竹提取物中部分纯化得到的有效化合物是一种分子量小于3000的还原性碳水化合物。该化合物对大肠杆菌GW1060（recA441）在42℃时发生的SOS反应具有抑制作用，但对大肠杆菌GW 1107（lexA51）中的SOS网络基因表达没有影响，这表明玉竹可能含有RecA蛋白酶的抑制剂。
