Jin Z C, Qian J
Department of Pathophysiology, Zhejiang Medical University, Hangzhou.
Zhonghua Yu Fang Yi Xue Za Zhi. 1994 May;28(3):147-50.
Effects of 15 kinds of herbal drugs, vegetables and chemicals on lex-dependent sfi-SOS response were determined by micropersistent and/or pulse models induced by 4-Nitroquinoline-N-oxide (4NQO) and Mitomycin C (MMC) in Escherichia coli(E. coli) PQ37 and PQ35, respectively. Results showed the water extract of Rhizoma Polygonati (RP), Fructus Chebulae (FC), Radix Polygoni Multiflori (RPM), Fructus Ligustri Lucidi (FLL), Bulbus Fritillariae Thunbergii (BFT), shell of water chestnut with a pedicle, Chinese chives juice, and solutions of 5-Fluorouracil, Tannic acid and garlicin could inhibit SOS responses with a dose-response relationship and suggested the inhibitory effects took place both inside and outside E. coli cells. Water extract of FC, FLL, BFT, shell of water chestnut with a pedicle, Chinese chives juice and solution of 5-Fluorouracil and Tannic acid could intracellularly inhibit SOS responses induced by MMC in E. coli PQ35, and acetone extract of Grifola Frondosa (GF) could extracellularly inhibit SOS responses in E. coli PQ37 and intracellularly in PQ35 induced by 4NQO or MMC. Water extract of raw hawthorn. Radix Angelicae Duhuricae (RAD), Radix Ophiopogonis (RO), and 5-Fluorodeoxyuridine could extracellularly inhibit SOS responses induced by 4NQO in E coli PQ37. The possible mechanisms of intracellular inhibition and antidamage repair were discussed in the paper.
分别通过4-硝基喹啉-N-氧化物（4NQO）和丝裂霉素C（MMC）诱导的大肠杆菌（E. coli）PQ37和PQ35中的微持续和/或脉冲模型，测定了15种草药、蔬菜和化学物质对Lex依赖性sfi-SOS反应的影响。结果表明，黄精（RP）、诃子（FC）、何首乌（RPM）、女贞子（FLL）、浙贝母（BFT）、带蒂菱角壳、韭菜汁以及5-氟尿嘧啶、鞣酸和大蒜素溶液的水提取物可抑制SOS反应，呈剂量反应关系，提示抑制作用在大肠杆菌细胞内外均有发生。FC、FLL、BFT、带蒂菱角壳、韭菜汁以及5-氟尿嘧啶和鞣酸溶液的水提取物可在细胞内抑制大肠杆菌PQ35中由MMC诱导的SOS反应，而灰树花（GF）的丙酮提取物可在细胞外抑制大肠杆菌PQ37中的SOS反应，并在细胞内抑制由4NQO或MMC诱导的PQ35中的SOS反应。生山楂、白芷（RAD）、麦冬（RO）的水提取物以及5-氟脱氧尿苷可在细胞外抑制大肠杆菌PQ37中由4NQO诱导的SOS反应。本文讨论了细胞内抑制和抗损伤修复的可能机制。
