猴病毒40晚期mRNA的5'末端序列和编码区源自病毒基因组的非连续片段。

5'-Terminal sequences and coding region of late simian virus 40 mRNAs are derived from noncontiguous segments of the viral genome.

作者信息

Lavi S, Groner Y

出版信息

Proc Natl Acad Sci U S A. 1977 Dec;74(12):5323-7. doi: 10.1073/pnas.74.12.5323.

DOI:10.1073/pnas.74.12.5323

PMID:202950

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC431702/

Abstract

The region of the simian virus 40 genome complementary to the 5' end of the most abundant poly(A)-containing 19S and 16S mRNAs was mapped by hybridization of double-labeled RNA ([3H]methyl group and [14C]uridine) to specific DNA fragments. Chemical identification of methylated residues indicated that a common "leader" sequence adjacent to the 5' terminus of both 19S and 16S mRNA is transcribed from DNA sequences located between 0.67 and 0.76 map units. The estimated size of this "leader" RNA, which does not code for any known viral protein, is 170-200 nucleotides. Our results indicate that sequences complementary to the "leader" region and coding portion of 16S mRNA are located in separate parts of the simian virus 40 genome.

摘要

通过将双标记RNA（[3H]甲基基团和[14C]尿苷）与特定DNA片段杂交，绘制了与最丰富的含多聚腺苷酸的19S和16S mRNA的5'端互补的猴病毒40基因组区域。甲基化残基的化学鉴定表明，与19S和16S mRNA的5'末端相邻的共同“前导”序列是从位于0.67至0.76图距单位之间的DNA序列转录而来的。这种不编码任何已知病毒蛋白的“前导”RNA的估计大小为170 - 200个核苷酸。我们的结果表明，与“前导”区域和16S mRNA编码部分互补的序列位于猴病毒40基因组的不同部分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13c9/431702/8875d1ce3017/pnas00043-0132-a.jpg

相似文献

5'-Terminal sequences and coding region of late simian virus 40 mRNAs are derived from noncontiguous segments of the viral genome.

Proc Natl Acad Sci U S A. 1977 Dec;74(12):5323-7. doi: 10.1073/pnas.74.12.5323.

Mapping the spliced and unspliced late lytic SV40 RNAs.

Cell. 1978 Aug;14(4):971-82. doi: 10.1016/0092-8674(78)90351-3.

Novel mechanism for RNA maturation: the leader sequences of simian virus 40 mRNA are not transcribed adjacent to the coding sequences.

Proc Natl Acad Sci U S A. 1977 Sep;74(9):3686-90. doi: 10.1073/pnas.74.9.3686.

Unspliced functional late 19S mRNAs containing intervening sequences are produced by a late leader mutant of simian virus 40.

Proc Natl Acad Sci U S A. 1981 Mar;78(3):1386-90. doi: 10.1073/pnas.78.3.1386.

Identification and mapping of N6-methyladenosine containing sequences in simian virus 40 RNA.

Nucleic Acids Res. 1979 Jun 25;6(8):2879-99. doi: 10.1093/nar/6.8.2879.

Sequence arrangement of the 5' ends of simian virus 40 16S and 19S mRNAs.

Proc Natl Acad Sci U S A. 1977 Nov;74(11):4982-5. doi: 10.1073/pnas.74.11.4982.

Mutational alterations within the simian virus 40 leader segment generate altered 16S and 19S mRNA's.

J Virol. 1979 Jan;29(1):209-19. doi: 10.1128/JVI.29.1.209-219.1979.

Mapping of transcription sites of simian virus 40-specific late 16S and 19S mRNA by electron microscopy.

Proc Natl Acad Sci U S A. 1977 Feb;74(2):496-500. doi: 10.1073/pnas.74.2.496.

Simian virus 40-rabbit beta-globin recombinants lacking late mRNA splice sites express cytoplasmic RNAs with altered structures.

J Virol. 1982 Apr;42(1):262-74. doi: 10.1128/JVI.42.1.262-274.1982.

DNA sequence analysis of simian virus 40 mutants with deletions mapping in the leader region of the late viral mRNA's: mutants with deletions similar in size and position exhibit varied phenotypes.

J Virol. 1981 Feb;37(2):730-7. doi: 10.1128/JVI.37.2.730-737.1981.

引用本文的文献

Simian virus 40-rabbit beta-globin recombinants lacking late mRNA splice sites express cytoplasmic RNAs with altered structures.

J Virol. 1982 Apr;42(1):262-74. doi: 10.1128/JVI.42.1.262-274.1982.

Polyoma virus infection of retinoic acid-induced differentiated teratocarcinoma cells.

J Virol. 1981 Jul;39(1):306-12. doi: 10.1128/JVI.39.1.306-312.1981.

DNA sequence analysis of simian virus 40 mutants with deletions mapping in the leader region of the late viral mRNA's: mutants with deletions similar in size and position exhibit varied phenotypes.

J Virol. 1981 Feb;37(2):730-7. doi: 10.1128/JVI.37.2.730-737.1981.

Simian virus 40 early mRNA's in lytically infected and transformed cells contain six 5'-terminal caps.

J Virol. 1981 Jan;37(1):7-16. doi: 10.1128/JVI.37.1.7-16.1981.

Leakage of nuclear transcripts late in simian virus 40-infected CV1 cells: quantitation of spliced and unspliced late 19S RNAs.

J Virol. 1980 Nov;36(2):595-600. doi: 10.1128/JVI.36.2.595-600.1980.

5,6-dichloro-1-beta-ribofuranosylbenzimidazole enhances premature termination of late transcription of simian virus 40 DNA.

Proc Natl Acad Sci U S A. 1980 Jun;77(6):3297-3301. doi: 10.1073/pnas.77.6.3297.

Topography of the three late mRNA's of polyoma virus which encode the virion proteins.

J Virol. 1980 Feb;33(2):637-51. doi: 10.1128/JVI.33.2.637-651.1980.

The synthesis and processing of a nuclear RNA precursor to rat pregrowth hormone messenger RNA.

Nucleic Acids Res. 1979 Jul 11;6(9):3133-44. doi: 10.1093/nar/6.9.3133.

Purification of specific adenovirus 2 RNAs by preparative hybridization and selective thermal elution.

Nucleic Acids Res. 1979 Feb;6(2):593-607. doi: 10.1093/nar/6.2.583.

On pre-messenger RNA and transcriptions. A review.

Mol Biol Rep. 1979 May 31;5(1-2):5-28. doi: 10.1007/BF00777484.

本文引用的文献

Isolation and characterization of simian virus 40 ribonucleic acid.

J Virol. 1972 Aug;10(2):193-201. doi: 10.1128/JVI.10.2.193-201.1972.

Capping of eucaryotic mRNAs.

Cell. 1976 Dec;9(4 PT 2):645-53. doi: 10.1016/0092-8674(76)90128-8.

Two adenovirus mRNAs have a common 5' terminal leader sequence encoded at least 10 kb upstream from their main coding regions.

Cell. 1977 Sep;12(1):9-21. doi: 10.1016/0092-8674(77)90181-7.

An amazing sequence arrangement at the 5' ends of adenovirus 2 messenger RNA.

Cell. 1977 Sep;12(1):1-8. doi: 10.1016/0092-8674(77)90180-5.

Translational control of protein synthesis.

Annu Rev Biochem. 1976;45:39-72. doi: 10.1146/annurev.bi.45.070176.000351.

Spliced segments at the 5' terminus of adenovirus 2 late mRNA.

Proc Natl Acad Sci U S A. 1977 Aug;74(8):3171-5. doi: 10.1073/pnas.74.8.3171.

Novel mechanism for RNA maturation: the leader sequences of simian virus 40 mRNA are not transcribed adjacent to the coding sequences.

Proc Natl Acad Sci U S A. 1977 Sep;74(9):3686-90. doi: 10.1073/pnas.74.9.3686.

Mapping of sequences with 2-fold symmetry on the simian virus 40 genome: a photochemical crosslinking approach.

Proc Natl Acad Sci U S A. 1977 Apr;74(4):1363-7. doi: 10.1073/pnas.74.4.1363.

Mapping of transcription sites of simian virus 40-specific late 16S and 19S mRNA by electron microscopy.

Proc Natl Acad Sci U S A. 1977 Feb;74(2):496-500. doi: 10.1073/pnas.74.2.496.

The arrangement of simian virus 40 sequences in the DNA of transformed cells.

Cell. 1976 Oct;9(2):269-87. doi: 10.1016/0092-8674(76)90118-5.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验

猴病毒40晚期mRNA的5'末端序列和编码区源自病毒基因组的非连续片段。

5'-Terminal sequences and coding region of late simian virus 40 mRNAs are derived from noncontiguous segments of the viral genome.

作者信息

出版信息

相似文献

引用本文的文献

本文引用的文献

文献AI研究员

用中文搜PubMed

文档翻译

Suppr 超能文献

猴病毒40晚期mRNA的5'末端序列和编码区源自病毒基因组的非连续片段。

5'-Terminal sequences and coding region of late simian virus 40 mRNAs are derived from noncontiguous segments of the viral genome.

作者信息

出版信息

相似文献

引用本文的文献

本文引用的文献