Namba Y, Tomonaga M, Kawasaki H, Otomo E, Ikeda K
Department of Neuropathology, Faculty of Medicine, University of Tokyo, Japan.
Brain Res. 1991 Feb 8;541(1):163-6. doi: 10.1016/0006-8993(91)91092-f.
During the course of our immunohistochemical studies on the change of lipids in Alzheimer's disease brains by using antibody to apolipoprotein E, a protein having a special relevance to nervous tissue, we unexpectedly found that apo E immunoreactivity was associated with amyloid in both senile plaques and cerebral vessels and neurofibrillary tangles. The immunoreactivity was also found in amyloid of kuru plaques in Creutzfeldt-Jakob disease. Pretreatment of the sections with formic acid greatly enhanced immunoreactivity of senile and kuru plaques to antibody to apo E.
在我们使用与神经组织有特殊关联的载脂蛋白E抗体对阿尔茨海默病大脑中的脂质变化进行免疫组织化学研究的过程中，我们意外地发现，载脂蛋白E免疫反应性与老年斑、脑血管和神经原纤维缠结中的淀粉样蛋白相关。在克雅氏病的库鲁斑淀粉样蛋白中也发现了这种免疫反应性。用甲酸预处理切片可大大增强老年斑和库鲁斑对载脂蛋白E抗体的免疫反应性。
