[携带黑麦-小麦易位的春性小黑麦品系131/7的分子细胞遗传学特征分析]

[Molecular cytogenetic characterization of spring triticale line 131/7 carrying a rye-wheat translocation].

作者信息

Divashuk M G, Krupin P Iu, Solov'ev A A, Karlov G I

出版信息

Genetika. 2010 Feb;46(2):211-7.

Abstract

The chromosomal composition of the spring triticale line 131/7 carrying a rye-wheat translocation was studied using GISH, C-banding, and SSR analysis. The complex analysis revealed the presence of a pair of 2D chromosomes and T2RS.2RL-2BL translocation in the genome of the hexaploid triticale line 131/7. The break point in the translocated chromosome is between the markers Xwmc592 and Xwmc441, loci 63.9 cM and 76.8 cM on the linkage map (Wheat, Consensus SSR, 2004 NA-SSR-2004-2B) and in the region C2BL2-0.36 on the physical map (Wheat, Physical, SSR). The analysis of the line enables its use in breeding programs and in genetic studies.

摘要

利用基因组原位杂交（GISH）、C 带分析和简单序列重复（SSR）分析，对携带黑麦 - 小麦易位的春性小黑麦品系 131/7 的染色体组成进行了研究。综合分析揭示了六倍体小黑麦品系 131/7 的基因组中存在一对 2D 染色体以及 T2RS.2RL - 2BL 易位。易位染色体上的断点位于标记 Xwmc592 和 Xwmc441 之间，在连锁图谱（小麦，共识 SSR，2004 NA - SSR - 2004 - 2B）上位于 63.9 cM 和 76.8 cM 位点，在物理图谱（小麦，物理图谱，SSR）上位于 C2BL2 - 0.36 区域。对该品系的分析使其能够应用于育种计划和遗传研究。

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