Ermakov A V, Kon'kova M S, Kostiuk S V, Smirnova T D, Kameneva L V, Veĭko R V, Kubasova I Iu, Liubchenko L N, Veĭko N N
Radiats Biol Radioecol. 2010 Jan-Feb;50(1):42-51.
Transposition and mutual approaching of pericentromeric loci 1q12 of homological chromosomes from the nuclear membrane towards the nuclear centre as well as activation of the chromosomal nucleolus-forming regions (NFR) are observed in human mesenchymal stem cells (hMSCs) as an initial stages of the adaptive response (AR) after exposure to low doses of X-radiation (10 cGy). All these reactions are also induced after addition of cultivation medium from irradiated cells to intact bystander-cells and this phenomenon called bystander effect (BE). Recently the same AR and BE induction results were obtained for human G0-lymphocytes. All these data indicate the existence of universal reaction of homological chromosome loci transposition which was revealed during AR development in differentiated (lymphocytes) and non-differentiated (hMSCs) and also it shows possibility of radiational BE development in suspension and monolayer cell cultures upon addition of stress-signalization factors in incubation medium. We suppose that these factors are extracellular genome DNA fragments apoptotic cells.
在人骨髓间充质干细胞（hMSCs）中观察到，同源染色体着丝粒周围区域1q12从核膜向核中心转位并相互靠近，以及染色体核仁形成区（NFR）激活，这是低剂量X射线辐射（10 cGy）后适应性反应（AR）的初始阶段。将辐照细胞的培养基添加到完整的旁观者细胞中后，也会诱导所有这些反应，这种现象称为旁观者效应（BE）。最近，在人G0淋巴细胞中也获得了相同的AR和BE诱导结果。所有这些数据表明存在同源染色体位点转位的普遍反应，这在分化细胞（淋巴细胞）和未分化细胞（hMSCs）的AR发展过程中被揭示，并且还表明在培养介质中添加应激信号因子后，悬浮和单层细胞培养中可能发生辐射性BE。我们推测这些因子是凋亡细胞的细胞外基因组DNA片段。
