Institute of Cell Biology, Zhejiang University School of Medicine, Hangzhou, PR China.
Clin Chem Lab Med. 2010 Jun;48(6):785-9. doi: 10.1515/CCLM.2010.154.
Toll-like receptor 2 (TLR2) is essential for the immune response to tuberculosis (TB). The goal of the present study was to investigate whether the guanine-thymine (GT) repeat microsatellite polymorphism in intron 2 of the TLR2 gene might be correlated with susceptibility to TB in Han Chinese.
The number of (GT)n repeats was determined by gene scanning from 244 patients with TB and 233 control subjects. The expression of TLR2 on CD14+ peripheral blood mononuclear cells was determined using flow cytometry.
No association in allelic polymorphism between control subjects and patients with TB was found. However, the S/M genotype of the microsatellite polymorphism was more frequent in TB patients than in healthy controls (p=0.01). The S/L genotype was more popular in controls than in patients with TB (p=0.007). TLR2 expression was higher in subjects with the S/L genotype than in those with the S/M genotype (p<0.05).
Our data suggest that the S/M genotype of the microsatellite (GT)n polymorphisms in intron 2 of the TLR2 gene may increase susceptibility to TB in Chinese, and the S/L genotype may act as a negative risk factor.
Toll 样受体 2（TLR2）对于结核病（TB）的免疫反应至关重要。本研究的目的是探讨 TLR2 基因内含子 2 中的鸟嘌呤-胸腺嘧啶（GT）重复微卫星多态性是否与汉族人对 TB 的易感性相关。
通过对 244 例 TB 患者和 233 例对照者进行基因扫描，确定（GT）n 重复的数量。使用流式细胞术测定 TLR2 在 CD14+外周血单个核细胞上的表达。
未发现对照组与 TB 患者之间等位基因多态性存在关联。然而，微卫星多态性的 S/M 基因型在 TB 患者中比在健康对照组中更为常见（p=0.01）。S/L 基因型在对照组中比在 TB 患者中更为流行（p=0.007）。S/L 基因型的 TLR2 表达高于 S/M 基因型（p<0.05）。
我们的数据表明，TLR2 基因内含子 2 中的微卫星（GT）n 多态性的 S/M 基因型可能增加中国人对 TB 的易感性，而 S/L 基因型可能作为一个负风险因素。
