Department of Diagnostic and Interventional Imaging, The University of Texas Medical School at Houston, Houston, TX 77030, USA.
Magn Reson Imaging. 2010 May;28(4):607-12. doi: 10.1016/j.mri.2010.01.004. Epub 2010 Mar 29.
Measurement of perfusion in longitudinal studies allows for the assessment of tissue integrity and the detection of subtle pathologies. In this work, the feasibility of measuring brain perfusion in rats with high spatial resolution using arterial spin labeling is reported. A flow-sensitive alternating recovery sequence, coupled with a balanced gradient fast imaging with steady-state precession readout section was used to minimize ghosting and geometric distortions, while achieving high signal-to-noise ratio. The quantitative imaging of perfusion using a single subtraction method was implemented to address the effects of variable transit delays between the labeling of spins and their arrival at the imaging slice. Studies in six rats at 7 T showed good perfusion contrast with minimal geometric distortion. The measured blood flow values of 152.5+/-6.3 ml/100 g per minute in gray matter and 72.3+/-14.0 ml/100 g per minute in white matter are in good agreement with previously reported values based on autoradiography, considered to be the gold standard.
在纵向研究中测量灌注可以评估组织完整性并检测细微的病变。在这项工作中，报告了使用动脉自旋标记以高空间分辨率测量大鼠脑灌注的可行性。采用流动敏感交替恢复序列，结合平衡梯度快速成像稳态进动读出部分，以最小化重像和几何变形，同时实现高信噪比。使用单次减影法进行灌注的定量成像，以解决自旋标记和到达成像切片之间的可变传输延迟的影响。在 7T 下对六只大鼠的研究显示出良好的灌注对比度，几何变形最小。在灰质中测量到的血流值为 152.5+/-6.3 ml/100 g/min，在白质中为 72.3+/-14.0 ml/100 g/min，与基于放射性自显影的先前报道的值非常吻合，放射性自显影被认为是金标准。
