Okabe Takafumi, Toda Takayoshi, Nukitrangsan Natthanan, Inafuku Masashi, Iwasaki Hironori, Yanagita Teruyoshi, Oku Hirosuke
United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima, Japan.
J Oleo Sci. 2010;59(4):161-7. doi: 10.5650/jos.59.161.
To optimize the adequate atherogenic diet composition for nutritional atherosclerosis studies utilizing B6.KOR-Apoe(shl) mice, we investigated the effect of dietary cholesterol, AIN formula, and dietary fats on the development of atherosclerosis. Cholesterol supplementation (0.15-2%) for 12 weeks resulted in a dose-dependent increase in the development of atherosclerosis. Furthermore, there were no significant differences in the degree of atherosclerosis between B6.KOR-Apoe(shl) mice fed a modified-AIN-76 diet and those fed a modified-AIN-93M diet containing corn oil or soybean oil for 10 and 12 weeks. The present experiment indicates that the adequate dietary level of cholesterol was 0.15%, and that further studies are necessary to determine the optimal level of various types of dietary oils for nutritional atherosclerosis experiments in B6.KOR-Apoe(shl) mice.
为了优化用于营养性动脉粥样硬化研究的致动脉粥样硬化饮食组成（使用B6.KOR-Apoe(shl)小鼠），我们研究了膳食胆固醇、AIN配方和膳食脂肪对动脉粥样硬化发展的影响。补充胆固醇（0.15 - 2%）12周导致动脉粥样硬化发展呈剂量依赖性增加。此外，喂食改良AIN-76饮食的B6.KOR-Apoe(shl)小鼠与喂食含玉米油或大豆油的改良AIN-93M饮食10周和12周的小鼠之间，动脉粥样硬化程度无显著差异。本实验表明，胆固醇的适宜膳食水平为0.15%，并且有必要进一步研究以确定B6.KOR-Apoe(shl)小鼠营养性动脉粥样硬化实验中各种类型膳食油的最佳水平。
