NSF Center for Biophotonics Science and Technology, University of California-Davis, Sacramento, CA 95817, USA.
J Biophotonics. 2010 Apr;3(4):216-23. doi: 10.1002/jbio.200900102.
Cell-cell interactions through direct contact are very important for cellular communication and coordination - especially for immune cells. The human immunodeficiency virus type I (HIV-1) induces immune cell interactions between CD4(+) cells to shuttle between T cells via a virological synapse. A goal to understand the process of cell-cell transmission through virological synapses is to determine the cellular states that allow a chance encounter between cells to become a stable cell-cell adhesion. We demonstrate the use of optical tweezers to manipulate uninfected primary CD4(+) T cells near HIV Gag-iGFP transfected Jurkat T cells to probe the determinants that induce stable adhesion. When combined with fast 4D confocal fluorescence microscopy, optical tweezers can be utilized not only to facilitate cell-cell contact, but also to simultaneously track the formation of a virological synapse, and ultimately to probe the events that precede virus transfer.
细胞间通过直接接触的相互作用对于细胞通讯和协调非常重要 - 特别是对于免疫细胞。人类免疫缺陷病毒 1 型（HIV-1）诱导 CD4(+)细胞之间的免疫细胞相互作用，通过病毒学突触在 T 细胞之间穿梭。了解通过病毒学突触进行细胞-细胞传递的过程的一个目标是确定允许细胞之间偶然相遇成为稳定的细胞-细胞粘附的细胞状态。我们展示了使用光学镊子操作未感染的原代 CD4(+)T 细胞靠近 HIV Gag-iGFP 转染的 Jurkat T 细胞，以探测诱导稳定粘附的决定因素。当与快速的 4D 共聚焦荧光显微镜结合使用时，光学镊子不仅可以促进细胞-细胞接触，还可以同时跟踪病毒学突触的形成，并最终探测病毒转移之前的事件。
