Shi Linda, Shao Bing, Chen Timothy, Berns Michael
UCI - Beckman Laser Institute, 1002 Health Sciences Road, Irvine, CA 92612, USA.
J Biophotonics. 2009 Mar;2(3):167-77. doi: 10.1002/jbio.200810053.
An automatic microscope system is designed to study the response of sperm motility to an annular laser trap. A continuous annular laser trap provides a parallel way to analyze and sort sperm based on their motility and to study the effects of laser radiation, optical force and external obstacles. In the described automatic microscope system, the phase contrast images of swimming sperm are digitized to the computer at video rates. The microscope stage is controlled in real-time to relocate the sperm of interest to the annular trap with a normal or tangential entering angle. The sperm is continuously tracked and the swimming behavior is identified. Using this system, parallel sorting on human and gorilla sperm are achieved and threshold power levels separating the "fast" group and the "slow" group are compared for those two species.
设计了一种自动显微镜系统，用于研究精子活力对环形激光阱的响应。连续环形激光阱提供了一种基于精子活力分析和分选精子以及研究激光辐射、光力和外部障碍物影响的并行方法。在所描述的自动显微镜系统中，游动精子的相差图像以视频速率数字化到计算机中。实时控制显微镜载物台，将感兴趣的精子以法线或切线入射角重新定位到环形阱中。持续跟踪精子并识别其游动行为。使用该系统，实现了对人类和大猩猩精子的并行分选，并比较了这两个物种中区分“快速”组和“慢速”组的阈值功率水平。
