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通过亲和色谱法制备纤维蛋白原选择性缺失的血浆。

Production of plasma selectively depleted in fibrinogen by affinity chromatography.

作者信息

Hirst C F, Stevenson K J

机构信息

United Kingdom Reference Laboratory for Anticoagulant Reagents and Control, Withington Hospital, Manchester.

出版信息

J Clin Pathol. 1991 Apr;44(4):306-8. doi: 10.1136/jcp.44.4.306.

DOI:10.1136/jcp.44.4.306
PMID:2030149
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC496904/
Abstract

An affinity chromatography column was prepared using a polyclonal anti-fibrinogen antibody bound to Sephacryl S 1000. Normal human plasma was depleted of fibrinogen by passage through this column. The fibrinogen free plasma corrected the prolonged clotting times of plasmas deficient in factors V, VII, VIII and IX. Clottable fibrinogen was recovered from the column. An immunodepleted fibrinogen free plasma could fulfil a useful role in internal and external quality control alone, or in combination with other test materials; it could also be used in research.

摘要

使用结合到Sephacryl S 1000上的多克隆抗纤维蛋白原抗体制备亲和层析柱。正常人血浆通过该柱后纤维蛋白原被去除。无纤维蛋白原血浆纠正了缺乏因子V、VII、VIII和IX的血浆延长的凝血时间。可从柱上回收可凝纤维蛋白原。免疫去除纤维蛋白原的无血浆单独或与其他测试材料结合可在内外部质量控制中发挥有益作用；它也可用于研究。

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本文引用的文献

1
[Rapid physiological coagulation method in determination of fibrinogen].[快速生理凝血法测定纤维蛋白原]
Acta Haematol. 1957 Apr;17(4):237-46. doi: 10.1159/000205234.
2
Immunodepletion of human plasma factor VIII.
Blood. 1980 Oct;56(4):604-7.
3
Preparation of factor VIII-free plasma by immunoaffinity chromatography on insolubilized antibodies against factor VIII-related antigen.通过在针对VIII因子相关抗原的固定化抗体上进行免疫亲和层析制备无VIII因子血浆。
Haemostasis. 1983;13(2):73-7. doi: 10.1159/000214706.
4
Preparation of factor VII-depleted plasma by immunoaffinity chromatography of insolubilized anti-factor VII.通过固定化抗凝血因子VII的免疫亲和色谱法制备缺乏凝血因子VII的血浆。
Med Lab Sci. 1986 Jul;43(3):215-9.
5
Human coagulation factor IX: direct depletion and recovery from plasma, using immobilised monoclonal antibody.人凝血因子IX：使用固定化单克隆抗体从血浆中直接去除和回收
Med Lab Sci. 1988 Jul;45(3):261-5.
6
Artificial factor VIII deficient plasma: preparation using monoclonal antibodies and its use in one stage coagulation assays.人工因子VIII缺乏血浆：使用单克隆抗体的制备方法及其在一期凝血试验中的应用。
J Clin Pathol. 1988 May;41(5):562-7. doi: 10.1136/jcp.41.5.562.
7
Production of factor VIII deficient plasma by immunodepletion using three monoclonal antibodies.使用三种单克隆抗体通过免疫去除法制备VIII因子缺乏血浆。
Br J Haematol. 1987 Aug;66(4):497-502. doi: 10.1111/j.1365-2141.1987.tb01334.x.
8
Reductive amination for solid-phase coupling of protein. A practical alternative to cyanogen bromide.用于蛋白质固相偶联的还原胺化反应。溴化氰的一种实用替代方法。
J Immunol Methods. 1986 Oct 23;93(1):83-8. doi: 10.1016/0022-1759(86)90436-9.
9
Factor VIII deficient plasma for laboratory tests prepared from normal plasma and a human antibody.用于实验室检测的VIII因子缺乏血浆，由正常血浆和一种人源抗体制备而成。
Haemostasis. 1977;6(3):157-62. doi: 10.1159/000214175.
10
Preparation of factor VIII-deficient plasma by immunoadsorption.
Vox Sang. 1979;36(6):342-6. doi: 10.1111/j.1423-0410.1979.tb04508.x.